Información General

• Autores: David Rodríguez Lázaro (ed. lit.)
• Editores: Caister Academic Press
• Año de publicación: 2013
• País: Reino Unido
• Idioma: inglés
• ISBN: 978-1-908230-15-7
• Títulos paralelos:
  • PCR en tiempo real en ciencia de los alimentos: tecnología y aplicaciones actuales
• Texto completo no disponible (Saber más ...)

Resumen

• Bacterial detection and control are vital aspects of food microbiology. Real-time PCR is one of the most significant advances in this area, providing rapid, reliable, and quantitative results. In recent years, real-time PCR has become increasingly important to the agricultural and food industries as a valuable alternative to traditional detection methods. The advantages of quantitative real-time PCR include speed, an excellent detection limit, selectivity, specificity, sensitivity, and the potential for automation. Written by experts in the field, this book is an indispensable manual for scientists in the food industry. The first section provides an introduction to real-time PCR, discusses the use of PCR diagnostics in food science, describes the principles and methods of sample preparation, and covers the verification and control of PCR procedures. The second section covers the use of real-time PCR to detect various pathogens including Salmonella, Listeria, E. coli, Campylobacter, Yersinia, Staphylococcus, Clostridium, viruses, and parasites. Also included is a chapter on the standardization of real-time PCR methods in food microbiology. In the final section, the book covers the use of real-time PCR for the analysis of genetically modified organisms, for food allergens, and for identification of animal or plant species. This will be an invaluable book for anyone involved in food microbiology or the detection of foodborne pathogens, and it is a recommended volume for all microbiology laboratories.

Otros catálogos

• Red de Bibliotecas Universitarias (REBIUN)

Listado de artículos

• • Real-time PCR Basics

    • Introduction to the Real-time Polymerase Chain Reaction

      David Rodríguez Lázaro, Marta Hernández Cabría

      • Resumen

      págs. 3-20

      

    • Current Challenges in Real-time PCR Diagnostics in Food Science

      David Rodríguez Lázaro, Nigel Cook, Marta Hernández Pérez

      • Resumen

      págs. 21-26

      

    • Sample Preparation for Real-time PCR in Food Science

      Tomáš Kuchta

      págs. 27-34

      

    • International Amplification Controls in Real-time Polymerase Chain Reaction-based Methods for Pathogen Detection

      Nigel Cook, Gabriel A. de Ridder, Martín D'Agostino, Maureen B. Taylor

      págs. 35-42

      

  • Food Microbiology

    • Standardization of Real-time PCR Methods in Food Microbiology

      Kornelia Berghof Jäger

      págs. 45-56

      

    • Real-time PCR Detection of Food-borne Pathogenic "Salmonella" spp.

      Burkhard Malorny, Dietrich Mäde, Charlotta Löfström

      págs. 57-78

      

    • Real-time PCR Methods for the Detection of "Listeria monocytogenes" in Foods

      David Rodríguez Lázaro

      • Resumen

      págs. 79-90

      

    • Real-time PCR Detection of Food-borne Pathogenic "Escherichia coli"

      Patricia Elizaquível Bárcenas, Gloria Sánchez Márquez, Rosa Aznar Novella

      págs. 91-116

      

    • Detectioin of Pathogenic Thermotolerant "Campylobacter" Species

      L. Jesús García Gil

      págs. 117-124

      

    • Detection of Pathogenic "Yersinia enterocolitica" by Real-time PCR in Foods

      Dietrich Mäde

      págs. 125-134

      

    • Real-time PCR Detection of Food-borne Pathogenic "Vibrio"

      Luciana Croci, Elisabetta Suffredini

      págs. 135-148

      

    • Real-time PCR Detection of Food-borne Pathogenic "Staphylococcus aureus"

      Beatrix Stessl, Martin Wagner

      págs. 149-154

      

    • Real-time PCR Detection of Food-borne Pathogenic "Clostridia"

      Kathie Grant, Corinne Amar

      págs. 155-172

      

    • Real-time PCR and Other Molecular Detection Methods for Food-borne Pathogenic viruses

      Doris Helen D'Souza, Marta Hernández Cabría, Nigel Cook, David Rodríguez Lázaro

      • Resumen

      págs. 173-188

      

    • Real-time PCR Detection of Food-borne and Water-borne Parasites

      George D. Di Giovanni, Gregory D. Sturbaum, Huw V. Smith

      págs. 189-214

      

  • Food Quality

    • Real-time PCR Analysis of Genetically Modified Organisms

      Arne Holst-Jensen

      págs. 217-238

      

    • Real-time PCR Analysis of Food Allergens and Gluten

      Maria Carmen Díaz Amigo, Bert Pöpping

      págs. 239-252

      

    • Real-time PCR Methods for Identification of Animal or Plant Species

      Barbara Brežná, Ľubica Piknová

      págs. 253-272