Real-time PCR Methods for the Detection of "Listeria monocytogenes" in Foods

• Autores: David Rodríguez Lázaro
• Localización: Real-time PCR in food science: current technology and applications / David Rodríguez Lázaro (ed. lit.), 2013, ISBN 978-1-908230-15-7, págs. 79-90
• Idioma: inglés
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• Resumen

  • Listeria monocytogenes is a bacterial pathogen that causes serious localized and generalized infections in humans. Traditional detection methods of this pathogen involves two enrichment steps and a final isolation in two specific culture media, and a final confirmation using biochemical and/or molecular techniques, and therefore more than 5 days are needed for a final confirmation. An alternative to accelerate results in diagnostics of this pathogen in food is the application of real-time PCR-based methods. They can provide highly selective results with a considerable reduction of the time for analysis. This new methodology has been implemented satisfactory in a wide spectrum of food categories including those more relevant to the transmission of this pathogen to humans.