A Novel Genetic Label for Detection of Specific Probiotic Lactic Acid Bacteria
- Autores: Leónides Fernández Álvarez, María Marín Martínez, Susana Langa Marcano, Rocío Martín Jiménez, Carlota Reviriego Herráez, Mónica Olivares Martín, Jordi Xaus, Juan Miguel Rodríguez Gómez
- Localización: Food science and technology international = Ciencia y tecnología de alimentos internacional, ISSN-e 1532-1738, ISSN 1082-0132, Vol. 10, Nº 2, 2004, págs. 101-108
- Idioma: inglés
- Títulos paralelos:
- Nuevo marcado genético para la detección de bacterias lácticas probióticas específicas
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Resumen
A novel method for genetic labelling of specific lactic acid bacteria strains was developed. The approach implied the transformation of the hosts with a plasmid containing a heterologous DNA fragment. The sequence of a DNA fragment that has been used to label a variety of genetically modified (GM) soya was used to design a forward primer and three reverse primers yielding PCR products recognisable by their sizes. Stability of the recombinant plasmid in the transformed strains was studied by PCR, and the results varied significantly depending on the strain. To test the usefulness of the DNA label to study in vivo properties of probiotic bacteria, such as viability after transit through the digestive tract, mice were orally inoculated with a genetically-labelled Enterococcus faecium strain. Later, their faeces were aseptically collected and the genetically-labelled strain was detected among the colonies that grew on MRS agar. (A)
