Fast, Affordable and multiplexed foodborne pathogen detection on miniaturized devices

• Autores: Sarah Gaspar Ferreira Azinheiro
• Directores de la Tesis: Jorge Barros Velázquez (dir. tes.), Marta Prado Rodríguez (dir. tes.), Alejandro Garrido Maestu (dir. tes.)
• Lectura: En la Universidade de Santiago de Compostela ( España ) en 2022
• Idioma: inglés
• Tribunal Calificador de la Tesis: Marta Lopez Cabo (presid.), Alexandre Lamas Freire (secret.), Kate Reddington (voc.)
• Programa de doctorado: Programa de Doctorado en Innovación en Seguridad y Tecnología Alimentarias por la Universidad de Santiago de Compostela
• Materias:
  • Ciencias médicas
    • Ciencias de la nutrición
      • Agentes patógenos de los alimentos
• Enlaces
  • Tesis en acceso abierto en: MINERVA
• Resumen

  • Food poisoning is a global public health concern affecting, not only developing, but also developed countries, where several pathogens are responsible for these foodborne diseases causing high number of hospitalization and death. The objective of this thesis was to develop an improved methodology for the multiplex detection of Salmonella spp., Shiga Toxin-producing E. coli (STEC) and L. monocytogenes, based in DNA detection, and its integration in a miniaturized device. To achieve this goal, the different steps of the analysis, including the sample pre-treatment, DNA amplification and visualization of the results were addressed in order to reduce the time of analysis, reduce the cost, and allow naked-eye detection. The methodology developed in this project allowed for the multiplex detection of the three targets, reducing the time of analysis to only 9 h vs 7 days by traditional approaches with similar sensitivity and specificity to qPCR. Furthermore, the possibility to integrate the amplification step in a miniaturize device opens the door for the development of Point-of-Care systems.