Lérida, España
‘Celeste’ cherry fruit were dipped in 3% (w/v) CaCl2 (2 min, room temperature) and then kept for 14 days at 0 °C, followed by 3 days at 20 ºC to simulate shelf life. Calcium treatment significantly decreased firmness loss and decay incidence, but did not affect weight loss. After storage, levels of the hemicellulose-containing cell wall fraction in treated cherries were similar to those in the controls, in spite of lower levels of EGase activity and expression of a fruit-associated EGase gene. However, after shelf life the opposite was found, expression levels in treated fruit overpassing those in the controls, with concomitantly lowered KOHsf yields. Transcript accumulation of an expansin, PaEXP1, was severely inhibited during cold storage regardless of treatment, but increased during the simulated retail period to levels similar to those at harvest, and was inversely correlated to fruit firmness.
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