Efecto del bicarbonato sobre la activación de la protein kinasa a (pka) y la fecundación in vitro en la especie porcina
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[1] Universidad de Murcia
Universidad de Murcia
Murcia, España
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- Localización: XVI Jornadas sobre Producción Animal: 19 y 20 de mayo de 2015, Zaragoza / Javier Álvarez Rodríguez (aut.), Begoña Panea Doblado (aut.), Jorge Hugo Calvo Lacosta (aut.), Mireia Blanco Alibés (aut.), José Alfonso Abecia Martínez (aut.), Daniel Villalba Mata (aut.), María Ángeles Latorre Górriz (aut.), Vol. 2, 2015, ISBN 978-84-606-7969-1, págs. 435-437
- Idioma: español
- Títulos paralelos:
- Effect of bicarbonate on protein kinase a (PKA) activation and on in vitro fertilization output in porcine
- Enlaces
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Resumen
Prior to their encounter with the oocyte, spermatozoa have to experiment some modifications to get the ability to fertilize the egg, the sperm capacitation. Some of the components of the oviductal fluid (such as HCO3-) play an essential role during sperm capacitation. This study attempts to apply the optimal HCO3-concentration during in vitro fertilization (IVF) process in porcine. First, the effect of different concentrations of HCO3-(0, 15 and 25 mM) on PKA substrates phosphorylation (indicative of capacitation) was evaluated in epididymal sperm; second, the IVF was performed with different HCO3 - concentrations in order to improve the efficiency of viable zygotes production. The results showed that 15 mM of HCO3 -produces a similar effect on sperm PKA activation (p>0.05) that when 25 mM of HCO3- was achieved. Moreover, when 15 mM of HCO3- was used in IVF medium a greater efficiency was observed compared to other groups. Although 15 mM group offers less penetration (0 mM: 0±0a, 15 mM: 79.61±2.81b, 25 mM: 97.42±1.14c; p<0.05), the monospermy was the highest (0 mM: 0±0a, 15 mM: 32.93±3.68b, 25 mM: 10.05±2.19c;p<0.05). These facts could indicate the necessity of readjust the HCO3- concentration in the current systems of sperm capacitation and IVF in swine.
