Efecto de las proteínas ezrina, hsp70-1a y hsp90į en la fecundación in vitro porcina
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[1] Universidad de Murcia
Universidad de Murcia
Murcia, España
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- Localización: XVI Jornadas sobre Producción Animal: 19 y 20 de mayo de 2015, Zaragoza / Javier Álvarez Rodríguez (aut.), Begoña Panea Doblado (aut.), Jorge Hugo Calvo Lacosta (aut.), Mireia Blanco Alibés (aut.), José Alfonso Abecia Martínez (aut.), Daniel Villalba Mata (aut.), María Ángeles Latorre Górriz (aut.), Vol. 2, 2015, ISBN 978-84-606-7969-1, págs. 438-440
- Idioma: español
- Títulos paralelos:
- Effect of ezrin, hsp70-1a and hsp90į proteins on porcine in vitro fertilization
- Enlaces
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Resumen
The prefertilization hardening of the zona pellucida (ZP) decreases polyspermic fertilization in pigs. Specific proteins responsible of that ZP changes are the osteopontin (SPP1) and oviductin (OVGP1). Recently, other oviductal factors have been identified as potential inducers of ZP hardening. Among these proteins are ezrin and heat shock proteins such as HSP70-1A and HSP90Į. However, its potential role on ZP hardening and further fertilization results have not been studied yet. This work was designed to test whether the incubation of in vitro matured pig oocytes with these proteins can harden the ZP and affect further fertilization results. Incubation of matured oocytes for 1 h in TALP medium supplemented with ezrin (200 ng/ml), HSP70-1A (500 ng/ml), HSP90Į (500 ng/ml) or without protein (control group) showed differences in ZP digestion time being higher in the ezrin group (113.9 ± 3.5 s; n=25) compared with control (66.0 ± 2.1 s; n=27), HSP70-1A (81.6 ± 2.8 s; n=25) and HSP90Į group (79.7 ± 3.8 s; n=25). Presence of ezrin during gametes coculture showed a significant decrease in penetrability and an increase on monospermy rate. The role of ezrin preventing sperm capacitation may explain these results but further studies are necessary to confirm this hypothesis.
