Bajo condiciones in vitro, la adhesión de los espermatozoides a las células del oviducto se ve facilitada si no tienen exteriorizada la fosfatidilserina

• R. López Úbeda ^[1] ; R. Diego ^[1] ; C. Matás ^[1]
  1. [1] Universidad de Murcia

     Universidad de Murcia

     Murcia, España

     
• Localización: XVI Jornadas sobre Producción Animal: 19 y 20 de mayo de 2015, Zaragoza / Javier Álvarez Rodríguez (aut.), Begoña Panea Doblado (aut.), Jorge Hugo Calvo Lacosta (aut.), Mireia Blanco Alibés (aut.), José Alfonso Abecia Martínez (aut.), Daniel Villalba Mata (aut.), María Ángeles Latorre Górriz (aut.), Vol. 2, 2015, ISBN 978-84-606-7969-1, págs. 364-366
• Idioma: español
• Títulos paralelos:
  • Under in vitro condition, spermatozoa without externalization of phosphatidylserine are most able to bind oviductal epithelial cells
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• Resumen

  • Phosphatidylserine translocation is a feature of apoptosis and a marker commonly used to study this phenomenon (Casao et al., 2010). Therefore, differences in the levels of PS translocation between the population of sperm bound and not bound to the oviductal epithelial cells (OEC) could be expected. The aim of this study was to characterize possible differences in the phosphatidylserine translocation of boar sperm membrane after the co-culture with OEC. Sperm cells treated by discontinuous gradient of isotonic Percoll (washed sperm; L) were added to the oviductal epithelial cells (OEC) and incubated for 30 minutes. As control, washed sperm was incubated on Petri dish without OEC (L30). Four different sperm samples were analyzed by Annexin V-Cy3™ Apoptosis Detection Kit (SigmaAldrich, Madrid, Spain) to differentiate between sperm cells with (Ann+)/without (Anní) inverted PS. Washed sperm (L) showed low levels of PS translocation (28.00%), these values increased (43.00%) after incubation in capacitation condition (TALP medium) without OEC (L30). Incubation with OEC resulted in two distinct groups: unbound sperm (NU) with high levels of PS inversion (94.00%) and bound sperm (U) that showed the lower levels (21.00%). These results showed that oviductal epithelial cells are able to select sperm cells without phosphatidylserine translocation and bind them.