Active Bowman–Birk inhibitors survive gastrointestinal digestion at the terminal ileum of pigs fed chickpea-based diets

• Autores: Alfonso Clemente, Elisabeth Jimenez, M Carmen Marin-Manzano, L. A. Rubio
• Localización: Journal of the science of food and agriculture, ISSN 0022-5142, Vol. 88, Nº 3, 2008, págs. 513-521
• Idioma: inglés
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• Dialnet Métricas: 1 Cita
• Resumen

  • BACKGROUND: Protease inhibitors of the Bowman–Birk family have been demonstrated to be naturally occurring chemopreventive agents in a wide range of in vitro and in vivo models. In vitro and in vivo experiments have reported that Bowman–Birk inhibitors (BBIs) may exert colorectal chemopreventive effects. To exert such effects, these proteins have to survive, at least to some extent, the digestive process within the gastrointestinal tract. RESULTS: In order to determine the survival rates of functional BBI proteins in vivo, five castrated male pigs (100 ± 2 kg body weight) fitted with T-shaped cannulas at the terminal ileum were fed a chickpea-based diet. Pigs fed hydrolysed casein as the only protein source were used as an experimental control diet. The survival rates of BBI proteins from chickpea-based diets at the terminal ileum, expressed in terms of trypsin (TIA) and chymotrypsin inhibitory activity (CIA), were 7.3 and 4.4%, respectively. The presence of BBI proteins in ileal samples from pigs fed chickpea-based diet was confirmed by SDS-PAGE and peptide mass fingerprinting. CONCLUSION: It is concluded that significant amounts of active BBI proteins reach the large intestine of the pig. Further pharmacological studies are necessary in order to determine the potential of dietary BBI proteins as colorectal chemopreventive agents in humans. Copyright © 2007 Society of Chemical Industry