Influence of the solvent system on the composition of phenolic substances and antioxidant capacity of extracts of grape (Vitis vinifera L.) marc

S. Agustín Salazar; L. A. Medina Juárez; H. Soto Valdez; F. Manzanares López; N. Gámez Meza

Ayuda

Influence of the solvent system on the composition of phenolic substances and antioxidant capacity of extracts of grape (Vitis vinifera L.) marc

S. Agustin-Salazar ^[1] ; L.A. Medina-Juárez ^[1] ; H. Soto-Valdez ^[2] ; F. Manzanares-López ^[2] ; N. Gámez-Meza ^[1]
1. [1] Universidad de Sonora
  
  Universidad de Sonora
  
  México
2. [2] Centro de Investigación en Alimentación y Desarrollo
  
  Centro de Investigación en Alimentación y Desarrollo
  
  México
Localización: Australian journal of grape and wine research, ISSN 1322-7130, Vol. 20, Nº 2, 2014, págs. 208-213
Idioma: inglés
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Resumen
- Background and Aims Grape marc (GM), a byproduct of winemaking, contains significant amounts of substances that are not efficiently used. This study investigated the effect of solvent systems on the extraction of phenolic substances and antioxidant capacity (AC) from the Carignan grape (Vitis vinifera L.) marc, as well as from the peduncle, skin and seed of Carignan.
  
  Methods and Results The solvent systems tested were (v:v): methanol (MeOH) : water (6:4, 7:3 and 9:1), MeOH : water : acetone (ACE) (3:3.5:3.5) and ethanol (EtOH) : water (6:4, 7:3 and 9:1). The highest extraction of phenolic substances and flavonoids was obtained with EtOH : water (6:4 and 7:3) and MeOH : water : ACE (3:3.5:3.5) followed by MeOH : water (7:3). The highest AC was obtained with MeOH : water : ACE (3:3.5:3.5) followed by EtOH : water (6:4 and 7:3). The concentration of phenolic substances varied from 4.72 to 7.05 g catechin equivalents (CE)/kg fresh mass (FM) for seed extracts, 2.72–3.55 g CE/kg FM for skin extracts and 2.03–2.11 g CE/kg FM for peduncle extracts. Catechin, epicatechin, rutin, myricetin, quercetin, epicatechin gallate, quercetin 3-O-glucuronide, quercetin 3-O-hexoside and malvidin 3-hexoside were found in the marc.
  
  Significance of the Study The best solvent systems for extracting phenolic substances with high AC from Carignan GM were MeOH : water : ACE (3:3.5:3.5) and EtOH : water (6:4 and 7:3). The main phenolic substances that remained in wine marc after fermentation were myricetin, catechin and epicatechin. The results of this study suggest that Carignan GM, considered an industrial waste, is a natural resource of antioxidants.