Información General

• Autores: Francesc Gòdia Casablancas (coord.), Martín Fussenegger (coord.)
• Editores: Springer USA
• Año de publicación: 2005
• País: Estados Unidos
• Idioma: inglés
• ISBN: 1-4020-2791-5
• Recoge los contenidos presentados a: European Society of Animal Cell Technology. General Meeting (18. 2003. Granada).
• Texto completo no disponible (Saber más ...)

Resumen

• The 18th ESACT meeting was celebrated in Granada (Spain) in May 2003, and was entitled "Animal Cell Technology Meets Genomics", in order to reflect that the emerging technologies in the area of genomics, proteomics and other "-omics"-type disciplines will provide key technological assets to increase knowledge and open new horizons in animal cell technology. During the meeting a variety of top-class emerging technologies were presented together with the lastest advances in more mature industrial areas. The meeting was opened by a first session devoted to the understanding of basic cellular mechanisms, and four sessions focused on applied aspects of animal cell technology: Cell-based therapies and gene-based therapies, target discovery and biopharmaceuticals. The Granada Meeting has also seen a special focus on forefront industrial case studies. The spirit and scientific excellence of the 18th ESACT meeting is now reflected in different chapters of the book. The book presents, in form of short papers, a high number of the contributions to the meeting, and has been prepared with the aim to provide a relevant reference of the current research efforts in Animal Cell Technology.

Otros catálogos

• Red de Bibliotecas Universitarias (REBIUN)

Listado de artículos

• • Cellular Mechanisms

    • Effect of Osmotic Pressure on Gs-Ns0 Expression System

      Mon Han Wu, George Dimopoulos, Athanasios Mantalaris, Julie Varley

      págs. 3-13

      

    • Molecular Approaches to Influence Epigenetic Effectors of Transient and Stable Transgene Expression in Mammalian Cells

      D. L. Hacker, M. Cotten, F. M. Wurm

      págs. 15-22

      

    • Genomic Exploration on Chinese Hamster Ovary Cells

      M. De Leon Gatti, K. Wlaschin, A. Rink, A. Sanny, K.S. Tan, P.M. Nissom

      págs. 23-29

      

    • Roles for Glycosylation in Receptor-Ligand Interactions in the Immune System

      Pauline M. Rudd, Anthony H. Merry, Raymond A. Dwek

      págs. 31-42

      

    • Investigations on Mannose-6-Phosphate Receptor Mediated Protein Uptake

      M. Dürrschmid, C. Jursik, N. Borth, R. Grabherr, O. Doblhoff Dier

      págs. 43-49

      

    • Analysis of Multiple Apoptosis Parameters Using a Microfluidic Chip-Based System

      M. Valer, T. Preckel, G. Luedke, C. Buhlmann

      págs. 51-57

      

    • Inhibiting Apoptosis in Cell Culture Using Multiple Inhibitors

      Bruno Figueroa, Eric Ailor, Mitchell Reff, J. Marie Hardwick, Michael Betenbaugh

      págs. 59-65

      

    • Pausing of Mammalian Cells by Cold Exposure, Limits and Opportunities

      L. Hunt, D. Hacker, H. El Abridi, F. Grosjean, M. Dejesus, M. Jordan

      págs. 67-70

      

    • The Functional Competence of Animal Cells: analysis of the Secretory Pathway

      Daniel E. Alete, Andrew J. Racher, John R. Birch, David C. James, C. Mark Smales

      págs. 71-74

      

    • Proteome Analysis of Recombinant CHO Cells Under Hyperosmotic Stress

      Moon Sue Lee, Kyoung Wook Kim, Young Hwan Kim, Gyun Min Lee

      págs. 75-78

      

    • Expression Profiling Analysis of Sodium Butyrate-Induced Chinese Hamster Ovary Cells in Defined Medium

      Mark W. Melville, Martin Sinacore, Louane E. Hann

      págs. 79-82

      

    • Intracellular Nucleotide Pools for Optimizing Product-Oriented Transient Transfection of Hek293 Cells in Suspension

      M.M. Bassani Molinas, C. Beer, F. Hesse, M. Wirth, Durocher G., A. Kamen

      págs. 83-86

      

    • Impact of Yeast Pyruvate Carboxylase on the Productivity of Animal Host Cell Lines

      M. Bollatifogolin, N. Irani, A.J. Beccaria, C. Schulz, J. Von Den Heuvel, C.B. Elias

      págs. 87-89

      

    • Environmental Effects of Lactate on High-Five™ Insect Cell Metabolism

      J. C. Drugmand, Y. J. Schneider, S.N. Agathos

      págs. 91-94

      

    • Correlation of Intracellular Nucleotide Pools to Amino Acid Concentrations in Culture Media by the Application of Multivariate Methods

      F. Hesse, A. Nelving, R. Wagner

      págs. 95-98

      

    • Engineering Trichoplusia ni (High-Five™) Insect Cells to Express a Cytosolic Pyruvate Carboxylase Enzyme Improves the Viability of the Cells in Batch, Fed-Batch and Perfusion Cultures

      M. C. Locas, C. B. Elias, S. Lanthier, L. Bisson, M. Perrier, A.A. Kamen

      págs. 99-102

      

    • Insect Cell Culture Medium Selection and Optimisation Based on Monitoring and Economical Considerations

      M. Lecina, A. Soley, P. Passamani, A. Casablancas, J. De Gràcia, C. Vela

      págs. 103-106

      

    • Antiapoptotic Activity of Synthetic and Nnatural Peptides

      František Franěk

      págs. 107-110

      

    • Effect of Antiapoptotic Genes Expression on Cell Growth and Monoclonal Antibody Productivity in a Hybridoma Cell Line

      S. Juanola, J. Vives, J. Andersen, E. Prats, L. Cornudella, J.J. Cairó

      págs. 111-113

      

    • Amplified Dicistronic Expression Units Mediate Apoptosis Protection in CHO-DG44 Cells Adapted for Growth in Serum-Free Media, Impact on Mitochondria Copy Number

      H. Meents, B. Enenkel, M. Fussenegger

      págs. 115-120

      

    • Effect of Reduced Water on the Apoptotic Cell Death Triggered by Oxidative Stress in Pancreatic β HIT-T15 Cell

      Y. P. Li, K. Teruya, Y. Katakura, S. Kabayama, K. Otsubo, S. Morisawa

      págs. 121-124

      

    • Characterization of Apoptosis in a CHO Cell Line Cultivated in Batch and Continuous Culture: effect of Medium, Specific Perfusion Rate and Chemical Inhibitors

      John Thrift, Glen Yamasaki, Rudiger Heidemann, Nada Abbas, Konstantin Konstantinov

      págs. 125-128

      

    • Hydrogen Peroxide-Induced Cellular Senescence is Regulated via Two Different Pathways

      Yoshizaki K., Katakura Y. I., Fujiki T., Tsunematu T., Teruya K., Shirahata S.

      págs. 129-131

      

    • Identification of Autocrine Factors Influencing Proliferation in Serum-Free Cultures of Trichoplusia NI Cells

      U. Eriksson, L. Häggström

      págs. 133-135

      

    • The Effects of Insulin and Long™R3IGF-I on Chinese Hamster Ovary Cells: cell Survival, Receptor Activation and Second Messenger Pathways

      C.A. Yandell, I.P. Butler, A.J. Sheehan, B.J. Wade, A.P. Simula, C. Goddard

      págs. 137-141

      

    • Conditioned Medium Factors in Protein-Free Cultures of NS0 Cells

      E. Spens, L. Häggström

      págs. 143-145

      

    • Production of Human Anti-Peptide Antibody for Clinical Use by In Vitro Immunization

      T. Tamura, Y. Katakura, M. Yamashita, Y. Aiba, S. Matsumoto, K. Teruya

      págs. 147-149

      

    • Optimization of in Vitro Immunization Protocol to Produce Antigen Specific Human Monoclonal Antibody by Demonstrating the Role of IL-10

      Q.H. Xu, Y. Katakura, M. Yamashita, K. Teruya, S.G. Fang, S. Shirahata

      págs. 151-153

      

    • Eextracellular Histone H4 from SF9 Cells Is Antimicrobial

      K. Calles, E. Lindskog, Å. Nossed, I. Svensson, L. Häggström

      págs. 155-157

      

    • Analyzing Calcium Phosphate Transfection of Adherent CHO Cells Using Microscopic Live Imaging

      F. Grosjean, M. Jordan, F. M. Wurm

      págs. 159-161

      

    • Effects of Multiplicity of Infection (MOI) and Cell Cycle on Baculovirus Infection Kinetics

      R. Haas, S. Reid, L.K. Nielsen

      págs. 163-165

      

    • Non-Freezing Preservation and Subsequent Recovery of Hepatocytes Under PO2- and pH-Controlled Conditions for Continuous Supply of Cells for Bioartificial Liver Support

      Kai IDing, Heino Büntemeyer, Jürgen Lehmann

      págs. 167-170

      

    • Selection of MDCK Subclones with Various Genotypic and Phenotypic Markers: sensitivity to Influenza Virus Infection

      Nicole Kessler, Ghislaine Roche, Audrey Vernet, Alexandra Erny, Christophe Abismil

      págs. 171-174

      

    • Extracellular Metalloproteases and Their Inhibitors Expressed by The IFN-γ Producing CHO-320 Cell Line

      J. Mols, F. Verhoeye, C. Peeters Joris, S.N. Agathos, Y. J. Schneider

      págs. 175-177

      

    • Novel Small-Molecule AKT1 Inhibitors Discovered by Redistribution™-Based High- Throughput Screening

      Morten Præstegaard, Søren Jensby Nielsen, Betina Kerstin Lundholt, Ivan Mikkelsen, Steven Loechel, Morten Heide

      págs. 179-181

      

    • Influence of Mistletoe Extracts and Its Components on in Vitro Physiology of Cancer Cells

      F. Sidler, T. Wermelinger, L. Rist, A. Hensel, A. Viviani

      págs. 183-185

      

    • Rapid in-Process Monitoring of Antibody Integrity, Purity and Titre- 2100 Bioanalyser (Lab on a Chip) Study

      Nesredin A. Mussa, Kee Y. Cheung, Imtiaz M. Alam, Steve Flatman

      págs. 187-192

      

    • Toxicological Evaluation of Haine River Waste-Water Samples to Appropriately Sensitized Cultured Fathead Minnow Cells Compared With the Microtox® Method

      P.J. Dierickx, C. van der Wielen

      págs. 193-196

      

  • Cell Based Therapies

    • 3D-Cultivation and Characterisation of Osteogenic Cells for the Production of Highly Viable Bone Tissue Implants

      M. Barthold, I. Majore, S. Fargali, F. Stahl, R. Schulz, S. Lose

      págs. 199-205

      

    • Carbon Source Has a Considerable Influence on Extracellular Matrix Formation, Demonstrated on Specific Collagen Expression of Primary Human Tenocytes

      D. Möbest, M. Jaeger, G. Hoben, D. Hamann, S. Wollner, G.B. Stark

      págs. 207-212

      

    • Design and Characterization of Cardiomyocyte-Derived Microtissues

      Jens M. Kelm, Elisabeth Ehler, Jean Claude Perriard, Martin Fussenegger

      págs. 213-219

      

    • Process Development for Standardized Generation of Monocyte-Derived Dendritic Cells: applicability in Breast Cancer Immunotherapy

      H. R. Bohnenkamp, T. Noll

      págs. 221-227

      

    • Stemline™ Hematopoietic Stem Cell Expansion Medium, a Serum-Free Medium for the Expansion of CD34+ Hematopoietic Stem Cells and Progenitors

      S.L. Leugers, D.W. Allison, B.J. Pronold, G. Rennebeck, G. Van Zant, J.D. Tario Jr.

      págs. 229-232

      

    • Mesenchymal Stem Cells: a Very First Step Towards Large Scale Cell Culture Strategies

      M. Bensellam, C. Bassens, M. Toallati, S. Lowagie, J. Werenne

      págs. 233-236

      

    • Expansion of Murine Embryonic Stem Cells on Microcarriers-Functional Characteristics and Scale-Up Potential

      Burg M., Quel G., Ruediger M., Zweigerdt R.

      págs. 237-240

      

    • In Vitro Cultivation of Rabbit Mesenchymal Stromal Cells on 3D Bioresorbable Calcium Phosphate Scaffolds for the Generation of Bone Tissue Implants

      S. Fargali, M. Barthold, M. Rohde, I. Majore, V. Jäger

      págs. 241-243

      

    • Establishment and Characterization of Human Medullary Thyroid Carcinoma Cell Lines for Immunotherapy

      G. Stadler, R. Voglauer, M. Wieser, H. Katinger, R. Pfragner

      págs. 245-248

      

    • Suppression of Cell Growth by Platinum Nanocolloids as Scavengers against Reactive Oxygen Species

      T. Hamasaki, T. Kashiwagi, S. Aramaki, T. Imada, T. Komatsu, Y. Li

      págs. 249-251

      

    • Comparison of Fluidized Bed and Fed Batch Reactor Cultures for Production of Anti-HIV-Antibody

      P. Hinterleitner, F. Unterluggauer, K. Landauer, D. Müller, R. Kunert, G. Blüml

      págs. 253-255

      

    • Suppression of Oxidative Stress-Induced Apoptosis of Neuronal Cells by Electrolyzed-Reduced Water

      T. Kashiwagi, T. Hamasaki, S. Kabayama, M. Takaki, K. Teruya, Y. Katakura

      págs. 257-260

      

    • Spheroid Formation by Encapsulation of Cancer Cells to Mimic Small Size Tumors

      A. Marc, E. Markvicheva, C. Jourdain, L. Bezdetnaya, J.L. Merlin, F. Guillemin

      págs. 261-263

      

    • Bioreactor for Continuous Biomechanical Characterization of Cellular Systems and Tissue-Engineered Biohybrid Tissues

      D. Möbest, M. Jaeger, J. Guttmann, M. Schneider, G.B. Stark

      págs. 265-267

      

    • Membrane-Separated Cocultivation of Cord Blood Hematopoietic Stem Cells with Stromal Cell Lines

      T. Fischbach, T. Noll

      págs. 269-271

      

    • Anti-Apoptotic Genes Protect an Insulinoma Cell Line From Cell Death Induced by Hypergl Ycemia

      Akiko Ogawa, Satoshi Terada, Masao Miki, Toshihisa Kimura, Akio Yamaguchi

      págs. 273-275

      

    • Preparation and Characterization of an Idiotype-Dendritic Cell Vaccing for Immunotherapy of Multiple Myeloma

      B. Opalka, P. Schütt, D. Brandhorst, U. Buttkereit, M. Tewes, T. Moritz

      págs. 277-280

      

    • Pretreatment with Polycations Enhances Adenoviral Infection Levels in Serum-Free PER.C6⊆ Cells as Well as Hek 293 Cells

      Padilla J., Burke V., Johnson J., Hartshorn J., Mcnorton S., Etchberger K.

      págs. 281-283

      

    • Optimizing the Production of Cardiomyocytes from Mouse Embryonic Stem Cells in a 2L Stirred Tank Reactor

      M. Schroeder, S. Niebruegge, A. Werner, R. Zweigerdt, M. Burg, J. Lehmann

      págs. 285-288

      

    • Establishment and Characterization of CD4+ Killer-T-Cells (KTCS) a Promising New Tool for Adoptive Immunotherapy

      R. Voglauer, C. Jursik, M. Prchal, H. Jungfer, J. Grillari, H. Katinger

      págs. 289-292

      

  • Gene Based Therapeutics

    • Successful Development of a Robust Adenovirus Production Process Primarily Relies on a Better Understanding of Packaging Cell Line Physiology and Vector Replication Kinetics

      A. Kamen, O. Henry, D. Jacob, Annie Bernier

      págs. 295-301

      

    • Retroviral Vector Stability: inactivation Kinetics and Membrane Properties

      P.E. Cruz, M. Carmo, A.S. Coroadinha, A. Bengala, D. Gonçalves, M. Teixeira

      págs. 303-308

      

    • Comparison of Host Cell Lines and Production Methods for a New Generation of Oncolytic Adenoviral Vectors

      Sean Forestell, Chris Celeri, Chris Dang, Tom Gong, Matthew Olsen, Inesse Sifi

      págs. 309-316

      

    • Process Development for a Veterinary Vaccine against Heartwater

      I. Marcelino, M. Sousa, C. Veríssimo, C. Peixoto, M.J.T. Carrondo, P.M. Alves

      págs. 317-320

      

    • A New Method for the Quantitative Determination of Enveloped Viral Particles

      C. Beer, M. Wirth

      págs. 321-323

      

    • Development of a 293T Cell Line for the Production of Virus Like Particles in Bioreactors

      Maria João L. Costa, Rui Malhó, João gonçalves, Guilherme N.M. Ferreia, Maria Raquel Aires Barros

      págs. 325-328

      

    • Effect of MOI and Medium Composition on Adenovirus Infection Kinetics

      Tiago B. Ferreira, Paula M. Alves, Délia Gonçalves, M.J.T. Carrondo

      págs. 329-332

      

    • Ion-Exchange Chromatography Based Strategies for Purification of Viral Vectors

      J. Vasi, R. Morenweiser, K. Eriksson, R. Lemmens, S. Herzer

      págs. 333-338

      

    • Scaling up of Endothelial Cells Culture for the Production of a Rickettsiae Vaccine: valuation of the Importance of the Interference Effects

      V. Hendrick, N. Kagye, Y. Kamba, S. Simon, A L. Thinsy, T. Marique

      págs. 339-341

      

  • Target Discovery

    • Inducible Gene Expression for Antibiotic Drug Discovery and Diagnostics

      Wilfried Weber, Nils Link, Manuela Spielmann, Bettina Keller, Cornelia C. Weber, Martin Fussenegger

      págs. 345-350

      

    • Nucleic Acid Capture Assay: a Novel High- Throuhgput Method for Direct Mrna Quantitation

      Goor J., Tsai. P., Wong A., Mehta S., Zheng L., Elliott L.O.

      págs. 351-357

      

    • High Throughput Transient Transfections of Hek293Ebna Cells — Devel Opment of an Integrated Expression Purification Process at 100 ml Scale

      Holger Heine, Laurence Rey, Christian Y. Arod, Jean Philippe Gaudry, Bruno Antonsson, Georg Feger

      págs. 359-364

      

    • Transient Gene Expression in Suspension Hek293 Cells: application to Large-Scale Protein Production

      L. Baldi, R. Jacquet, S. Picasso, P. Tromba, E. Derow, P. Girard

      págs. 365-367

      

    • Monoclonal Antibody Production: Determination of Appropriate System and Medium for Production Enhancement and Decrease of Overall Production Time

      K. Fritchman, C. Tilsaghani, T. Sumnall

      págs. 369-371

      

    • The Secrets of Transfection in Serumfree Suspension Culture

      S. Geisse, N. Di Maiuta, B. Ten Buren, M. Henke

      págs. 373-376

      

    • From Genes to Proteins: full Open Reading Frame (ORF) Clones and Their Application in Functional Research

      A. Goedde, S. Baars, K. Czerny, E. Schultz, N. Gernold, T. Scheuermann

      págs. 377-379

      

    • A Versatile Disposable Culture System for High Throughput Screening of Process Parameters and Production Cell Lines

      M. Jordan, M. J. De Jesus, C. Eigenmann, T. Gleich, F. M. Wurm

      págs. 381-383

      

    • Long Time Storage of Pre-Inoculated Multi-Well PLlates for Cell-Based Assay Kits Used for Drug Iedntification and Characterisation

      Alexander Loa, Claudia Gelli, Bernd Laffert

      págs. 385-387

      

    • Comparative Secretion Levels of Recombinant Proteins between Insect Cell-Bbaculovirus and Mammalian Hek293EBNA Cells

      Christophe Losberger, Thierry Bbattle

      págs. 389-391

      

    • Microfluidic Technology Applied to Protein Sizing and Quantitation

      M. Valer, T. Neumann, P. Barthmaier, M. Kuschel, C. Buhlman

      págs. 393-396

      

    • Cryostock a Software for Cell Culture Management

      Marion Zerlin, Lars Greiffenberg, Yannick Wurm, Irvin Winkler, Dieter Kreusel, Stefan Toemoe

      págs. 397-400

      

  • Biopharmaceuticals

    • Targeting the Human Immunoglobulin Loci for High Level Hgene

      K. Winkler, T. Wermelinger, C. Paul, S Koch, R. Brecht, S. Zietze

      págs. 403-409

      

    • Mar Elements as Tools to Increase Protein Production by CHO Cells

      P. A. Girod, M. M. Zahn Zabal, N. Mermod

      págs. 411-415

      

    • Cell Line Engineering for Production of Therapeutic Antibody Glycoforms with Increased Biological Activity

      Pablo Umana, Peter Bruenker, Claudia Ferrara, Samuel Moser, Tobias Suter, Christian Gerdes

      págs. 417-422

      

    • Development of a Metabolically Optimized Fermentation Process Based on Glucose-Limited CHO Perfusion Culture

      Link T, Essers R, Zörner K, Gätgens J, Graaf De A, Backstrom M

      págs. 423-430

      

    • Applications of Quasi Real-Time Metabolic Flux Analysis in Mammalian Cell Culture Process Development

      Chetan Goudar, Richard Biener, James Michaels, Chun ZHang, James Piret, Konstantin Konstantinov

      págs. 431-438

      

    • Data-Based Modelling of Cell Cultures

      C. Cunha Bakeev, J. Glassey, G. Montague, M. Al Rubeal, P. Hardwicke

      págs. 439-445

      

    • Cell Culture and Downstream Processing Integration Using Proteinchip® Technology Application to Protein Analysis and Purification in Biopharmaceutical Manufacturing

      S. Bengio, P. Santambien, S. Cleverley

      págs. 447-452

      

    • The Prospects for Insect Cells for Use as a Cell Substrate for the Production of Biotherapeutics

      D.N. Galbraith

      págs. 453-458

      

    • High Performance Cell Culture Platform Technology for MAb Production

      David Y.H. Chang, Paul Garza, Yao Ming Huang, Mylene Talabardon, Soheil Rahmati, Eric Fallon

      págs. 459-464

      

    • Development and Integration of a New Animal-Component-Free Process for the Production of UK-279,276

      S. Pluschkell, L. Blocker, R. Geldart, S. Hawrylik, P. Mensah, C. Okediadi

      págs. 465-470

      

    • Towards an Affinity-Based Capture System for the Isolation of High Expression Cells Using a Co-Expressed Surface Protein

      M. Derouazi, H.M. Pick, C. Deluz, S. Picasso, R. Jacquet, F.M. Wurm

      págs. 471-473

      

    • Effect of Doxycycline-Regulated Calnexin and Calreticulin Expression on Specific Thrombopoietin Productivity of Recombinant CHO Cells

      Joo Young Chung, Seung Wook Lim, Yeon Joo Hong, Yeo Wook Koh, Sun Ok Hwang, Gyun Min Lee

      págs. 475-478

      

    • Recombinant Glycoprotein Expression Development of a Homologous CHO Expression System

      Noone C.B., Smith T.J., Cairns M.T.

      págs. 479-482

      

    • Generation of Recombinant Antibody Manufacturing Cell Lines Using Cell-Cell Fusion

      Wenlin Zeng, Ela Puchacz, Katy Heineken, Isaac Raymond, Conway Chang, Thomas Ryll

      págs. 483-488

      

    • Cloning and Expression of Biopharmaceuticals in CHO.K1 and BHK-21 Cells

      G.F. Asensi, J.M. Cavalcanti, P.M. Sousa, E.M. Del Aguila, J.T. Silva, V.M.F. Paschoalin

      págs. 489-492

      

    • Camel Single-Domain Antibodies as Modular Building Blocks to Make Bivalent Constructs for Use in Immunotherapy of Cancer

      Virna Cortez Retamozo, N. Backmann, P. Senter, U. Wernery, P. De Baetselier, S. Muyldermans

      págs. 493-495

      

    • Establishment of Efficient Cloning Method for Variable Region Genes of Antigen Specific Human Monoclonal Antibody

      S. Matsumoto, Y. Katakura, M. Yamashita, Y. Aiba, E. Noguchi, K. Teruya

      págs. 497-499

      

    • From Gene to Monoclonal Antibody: efficient Screening by Cell Sorting

      Borth N., Böhm E, Grillari J., Löscher M., Gross S., Voglauer R.

      págs. 501-504

      

    • Development of an Integrated Strategy for Recombinant Cell Line Selection

      A. J. Castillo, S. Víctores, E. Faife, Y. Rabasa, K. R. De Laluz

      págs. 505-507

      

    • Gene Transfer to a Animal Cells in Culture by Microinjection: a Novel Tool to Create Recombinant Cell Lines?

      M. Derouazi, P. Girard, M. Jordan, C. Denoya, F.M. Wurm

      págs. 509-512

      

    • Evaluation of the Stability of NS0 Cell Lines Expressing Recombinant Human IgG

      Wyn Forrest Owen, Lekan Daramola, Diane Hatton, Ray Field

      págs. 513-515

      

    • High Throughput Screening and Selection of Single Cells in Suspension Using Fluorescence Parameters

      C. Giese, C.D. Demmler, G. Gradl, U. Marx

      págs. 517-520

      

    • Control of Key Parameters in the Development of Mammalian Production Clones

      R. Kunert, S. Wolbank, M. Chang, S. Preis, W. Steinfellner, N. Borth

      págs. 521-524

      

    • Establishment of Screening Systems for Recombinant Cell Lines: early Prediction of the Suitability as a Production Clone

      Ch. Lattenmayer, R. Kunert, H. Katinger

      págs. 525-527

      

    • Characterisation of CHO Subclones Showing Profound Changes in Performance When Propagated in Different Cultivation Systems

      E. Trummer, D. Müller, W. Steinfellner, R. Kunert, F. Steindl, F. Hesse

      págs. 529-532

      

    • High Level Production of Recombinant IgG in the Human Cell Line PER.C6™

      C. Yallop, M. Raamsman, M. Zuijderwijk, Y. Van Noordenburg, A. Vooys, R. Keehnen

      págs. 533-536

      

    • Creating a New Medium to Help Meet the Variable Nutritional Requirements of Chinese Hamster Ovary (CHO) Cell Clones

      Z. Deeds, A. Albee, B. Delong, J. Gifford, S. Ross, K. Kao

      págs. 537-540

      

    • Development of an Efficient Medium Optimization Kit for Factorial Matrix Design—A Statistical Approach to Increase Cell Growth and Productivity of Recombinany CHO Cells

      B. Delong, A. Albee, Z. Deeds, J. Gifford, S. Ross, K. Kao

      págs. 541-547

      

    • An Efficient Approach to Cell Culture Medium Optimization — A Statistical Method to Medium Mixing

      Gifford J., Albee A., Deeds ZW., Delong B., Kao K., Ross S.

      págs. 549-553

      

    • Virus Inactivation in Foetal Calf Serum by a Combined Treatment of Gammairradiation and UV-C Irradiation

      Hermann H., Burian R., Waldmann R.

      págs. 555-559

      

    • Advances in Media Optimization: two Automated Approaches that Increase Expression While Reducing Development Time

      Stacy Holdread, Cindy Hunt, Perry Haaland, Bryce Chaney, Warren Porter, Mohammad Heidaran

      págs. 561-563

      

    • The Importance of Cholesterol for Insect Cell Growth and Baculovirus Production

      Linda H.L. Lua, Steven Reid

      págs. 565-568

      

    • Use of Rapeseed Proteins and Peptides as Supplements in Insect Cell Cultures

      A. Marc, V. Deparis, C. Durrieu, I. Marc, J.L. Goergen, I. Chevalot

      págs. 569-571

      

    • Nutritional Performance of Wheat-Gluten-Derived Protein Hydrolysates in Hybridoma Cell Cultures: a Nutritional Function Study

      Dirk E. Martens, Hendrikus B.A. Wegkamp, Nicholas H. Simpson, Hans H. Huttinga, Ton Kunst, André D. Siemensma

      págs. 573-575

      

    • Synthechol™ Ynthetic Cholesterol for Cholesterol Dependent Cell Culture — Development of Non-Animal Derived Chemically Defined NS0 Medium

      Talley D., Cutak B., Rathbone E, Kolla T., Allison D., Blasberg J.

      págs. 577-580

      

    • NS0 Derivatives: MAB Production in Largescale SFM Formats

      Manwaring J., Barnett B., Pence B., Whitford W.

      págs. 581-584

      

    • Silk Protein Sericin Accelerates Proliferation of Various Mammalian Cells

      Satoshi Terada, Kana Yanagihara, Kozue Kaito, Masao Miki, Masahiro Sasaki, Kazuhisa Tsujimoto

      págs. 585-587

      

    • Image Analysis Based Real Time-Control of Glucose Concentration

      T. Brueckerhoff, J. G. Frerichs, K. Joeris, K. Konstantinov, T. Scheper

      págs. 589-592

      

    • Online Analysis of Microcarrier Cultivations

      Arne Burzlaff, Cornelia Kasper, Thomas Scheper

      págs. 593-595

      

    • Error Analysis during Estimation of Metabolic Fluxes through Metabolite Balancing

      Chetan Goudar, Richard Biener, James Michaels, James Piret, Konstantin Konstantinov

      págs. 597-600

      

    • Generalized Logistic Equation Modeling of Mammalian Cell Batch Cultures

      Chetan Goudar, Rüdiger Heidemann, Klaus Joeris, James Michaels, James Piret, Konstantin Konstantinov

      págs. 601-604

      

    • In-Situ Microscopy Based Monitoring of Mammalian Cell Culture Processes

      Klaus Joeris, Jan Gerd Frerichs, Thomas Scheper, Konstantin Konstantinov

      págs. 605-608

      

    • A Noel Disposable Microtube for Rapid Assessment of Biomass in Cell Cultures

      Martin Jordan

      págs. 609-612

      

    • Intracellular Monitoring of Baculovirus Infection of SF9 Insect Cells and R-Protein Production Using Anti-Alpha-1,3/4 Fucosyl Transferase Antibodies and Flow Cytometry

      A. Marc, V. Deparis, A. Jeston, J. L. Goergen

      págs. 613-616

      

    • Effect of Glucose and Glutamine Concentration on Metabolism of Animal Cells in Chemostat Culture

      H. Matsuoka, T. Takeda

      págs. 617-620

      

    • Evaluation of a Novel apacitance Probe for On-Line Monitoring of Viable Cell Densities in Batch and Fed-Batch Animal Cell Culture Processes

      G. Schmid, D. Zacher

      págs. 621-624

      

    • Sodium Butyrate Enhancement of Protein Expression in Hela Cells: follow-Up of a Chimeric Reporter Gene Behaviour for Process Development

      C. Bassens, P. Rigaux, V. Hendrick, M. Cherlet, K. Sato, K. Kotarsky

      págs. 625-627

      

    • Scalable, Serum-Free, High Efficiency Transfection of CHO Cells Using an Economical DNA Transfer Vehicle

      P. Girard, M. Derouazi, F. Van Tilborgh, F.M. Wurm

      págs. 629-632

      

    • Process Optimization for an NS0-Derived Hybridoma Cell Line in a Chemically Defined, Protein-Free Medium

      J. M. Johnson, J. Hartshorn, S. Mcnorton, J. A. Padilla, T. Mohabbat, S. Luo

      págs. 633-636

      

    • Impact of Change in Fermentation Process pH and Dissolved Carbon Dioxide Concentration on Secreted Antibody Structure and Cell Physiology of a GS-NS0 Cell Line Expressing a Human Antibody

      Alison Ridley, Jonathan Dempsey, Chris Gee, Richard Turner, Matthew Osborne, Steve Ruddock

      págs. 637-640

      

    • Experimental Factorial Design for the Study of Some Variables in Roller Bottles Cell Culture

      Rodríguez E.N., Pérez M., Ordaz Y, Casanova P.R., L. Martinez, Herrera N.

      págs. 641-643

      

    • Evaluation of Rollercell System for High Scale Production of Biopharmaceuticals

      Rodríguez E.N., Pérez M., Ordaz Y, Casanova P.R., L. Martinez, Herrera N.

      págs. 645-647

      

    • Strategies for the Process Development of Monoclonal Antonpdoes for Therapeutic Use

      Mattei C., Dt Bernardi N., Gaspar Fernández San Elías, Castiglioni S., Orlandi A., Muru E

      págs. 649-653

      

    • Optimisation of PEI-Mediated Transient Expression in Chinese Hamster Ovary Cells

      Andrew S. Tait, Mike Hoare, John Birch, Douglas J. Galbraith, MIchael Hines, Catherine J. Brown

      págs. 655-657

      

    • Development of Fed-Batch Process Producing Monoclonal Antibodies Using In-House Media

      Yao Ming Huang, Thu Huynh, Le Ly, Wolfgang Noe, David Y. H. Chang

      págs. 659-661

      

    • Production of an Anti β2 Integrin Monoclonal Antibody by a Hybridoma Cell Line Grown in a 2 Liter Bioreactor

      Kallel H., Bellila A., Fathallah D. M.

      págs. 663-666

      

    • Economic Evaluation of Disposables vs Stainless Steel within a Modular Pilot Plant Facility — a Comparative Analysis

      Miriam Monge, Andrew Sinclair

      págs. 667-670

      

    • CGMP Manufacturing of a Fusion Protein in Mammalian Cells Using a Large-Scale Acoustic Perfusion System

      John Crowley, Carina Schlukebir, Maikel Dijkstal, Suzan Hoeksma, Edith Olthof, Stefanie Hußmann

      págs. 671-674

      

    • Influence of Culture Parameters on Viability in Perfusion Process

      Mcf Dalm, Smr Cuijten, Wmj Van Grunsven, A Oudshoorn, J Tramper, De Martens

      págs. 675-677

      

    • Use of Hydrocyclone as an Efficient Tool for Cell Retention in Perfusion Cultures

      E.A. Elsayed, G. W. Piehl, J. Nothnagel, R.A. Medronho, W. D. Deckwer, R. Wagner

      págs. 679-682

      

    • Effect of Different Variables on the Long-Term Spinfilter Clogging during Pilot-Scale Animal Cell Perfusion Runs

      Figueredo A., Alberto de Jesús Navarrete, Vitón P., Martínez E, Castro A., E. Chico

      págs. 683-685

      

    • Integrating Acoustic Perfusion in Mammalian Cell Culture: Temperature Control

      T. Keijzer, F. Trampler, A. Oudshoorn, H. V/D Bberg

      págs. 687-691

      

    • Flexible Fed-Batch Process Control of Animal Cells

      B. Frahm, R. Pörtner

      págs. 693-695

      

    • Development of a Fed-Batch Strategy for the Production of NS0-Derived Humanised Mab in Protein Free Medium

      Ojito Magaz E., Arias M.A., Chico Veliz E.

      págs. 697-700

      

    • Transfection to Manufacturing: reducing Timelines for High Yielding GS-CHO Processes

      M. Rendall, A. Maxwell, D. Tatham, P. Khan, R.D. Gay, R.C. Kallmeier

      págs. 701-704

      

    • Optimization of a Fed-Batch Process Producing Humanized Antibodies

      Paul Sauer

      págs. 705-709

      

    • Optimized Feeding Strategy for NS0 Cells

      Jennifer Walowitz, Lia Tescione, William Paul, David Jayme, Stephen Gorfien

      págs. 711-714

      

    • Comparison of Complementary Interactions between Synthetic Ligands and t-PA by Hpmdc

      Alexander Tappe, Gerlinde Kretzmer, Cornelia Kasper, Thomas Scherer, Evgenia G. Vlakh, Tatiana B. Tennikova

      págs. 715-717

      

    • Effect of Nutrient Supplementation on the Biological Quality of tPA Produced by CHO Cells on Serum-Free Medium

      C. Altamirano, A. ILlanes, R. Canessa, S. Becerra, J. Berrios

      págs. 719-722

      

    • Modulation of the Glycosylation Repertoire of a Recombinant Human EPO Expressing Model Cell Line Under Different Culture Conditions

      S. Duvar, J. Berlin, H. Ziehr, H.S. Conradt

      págs. 723-725

      

    • Physicochemical Estimation of rhEPO Potency Produced in Suspension Culture

      M. Etcheverrigaray, G.I. Amadeo, C. Didier, D. A. Pereira Bacci, F. A. Cavatorta, R. Kratje

      págs. 727-729

      

    • Post-Translational Modification of Recombinant Human GM-CSF Expressed by Stable-Transfected CHO Cells under Different Culture Conditions

      A.G. Forno, M. Etcheverrigaray, M. Nimtz, R.B. Kratje

      págs. 731-734

      

    • Softmix, a new Scalable Mixing System for the Cultivation of Sensitive Cells on Microcarrier under Protein- Free Conditions

      S. Naschberger, K. Rütten, D. Müller, H. Katinger

      págs. 735-738

      

    • Establishment of Fermentation Process for Hrepo Using Stirred Tank Fermenters in Perfusion Mode Using A Commercial Protein-Free Culture Medium

      Bouzó L., Ojito E, Arias M.A., Suarez J., Chico E, A. Alvarez

      págs. 739-742

      

    • Continuous Gmp Production in Hollow Fibre Bioreactors — Aviable Alternative for the Production of Low Volume Biopharmaceuticals

      R. Brecht, H. Bushnaq Josting, S. Zietze, R. Nuck, C. Kloth, A. Schwadtke

      págs. 743-745

      

    • Optimizing Cultivation Strategies in Different Scales of Hollow Fiber Bioreactors

      Gerardo Rodríguez Sánchez, M.A. Arias, Jesús Suárez de la Fuente, M. Chea, L. Bouzó, R. Cuervo

      págs. 747-750

      

    • Increasing Antibody Production from Hollow Fiber Systems

      Michael J. Gramer

      págs. 751-753

      

    • Production of Poliovirus, Rubella Virus, Measles Virus and Recombinant Adenovirus in Protein-Free Media

      Cory J. Card, Thomas Smith, Brent Hunsaker, Bill Barnett

      págs. 755-759

      

    • Serum-Free Production of Poliovirus: a Comparative Study Using Microcarriers, Roller Bottles and Stationary Cell Culture

      Cory J. Card, Thomas Smith, Brent Hunsaker, Bill Barnett

      págs. 761-765

      

    • Glutamine-Free Media for Vaccine Production Processes

      Y. Genzel, R. Alt, U. Reichl

      págs. 767-770

      

    • Serum Free Cultivation of Primary Chicken Embryo Fibroblasts in Microcarrier Systems for Vaccine Production

      B. Hundt, A. Schänzler, U. Reichl

      págs. 771-774

      

    • Design of an Animal Protein Free Medium to Sustain Vero Cells Growth in a Stirred Tank Bioreactor

      Kallel H., Rourou S., Arkadiy A. Skvortsov, Thalen M., Velden De Groot T.

      págs. 775-778

      

    • Use of On-Line Our Measurements to Monitor and Improve Insect Cell Cultures for Recombinant Protein Production

      M. Lecina, Antoni Casablancas Mira, A. Soley, C. Vela, Antoni Espuña Camarasa, J. De Gràcia

      págs. 779-782

      

    • Production of Live Influenza Vaccine in MDCK Cell Line

      N. Mazurkova, E. Nechaeva, T. Ryabicheva, A. N. Varaksin, T. Sen'Kina, M. Sviridenko

      págs. 783-785

      

    • Elaboration of the Microencapsulated Forms of the Viral Vaccines

      E. Nechaeva, A. N. Varaksin, T. Ryabicheva, T. Getmanova, N. Mazurkova, T. Senkina

      págs. 787-789

      

    • HTS-Based Development of High-Producing CHO Cell Lines

      K. Sautter, J. Fieder, R. Otto, B. Enenkel

      págs. 791-792