Effects and mechanisms of water extract of Uraria crinita on manganese chloride-induced apoptosis in SH-SY5Y cells

• Autores: Liang Jiefei, Yin Weiwei, Li Duanzhuo, Huang Mi, Si Wenxia, Luo Chao, Wu Wei Bin
• Localización: CyTA: Journal of food, ISSN 1947-6337, ISSN-e 1947-6345, Vol. 23, Nº. 1, 2025
• Idioma: inglés
• Enlaces
  • Texto completo
• Resumen

  • Uraria crinite (UC), a medicinal food commonly used in China, has been demonstrated to possess neuroprotective properties against oxidative stress. However, its potential to achieve neuroprotection by inhibiting endoplasmic reticulum (ER) stress remains unverified. This study employed a manganese chloride (Mncl2)-induced SH-SY5Y cells model to simulate ER stress-mediated neurodegeneration and investigated whether UC can exert neuroprotective effects by alleviating ER stress. The results showed that within the concentration range of 50–200 μg/ml, water extract of UC inhibited Mncl2-induced ER stress, oxidative stress, and autophagy levels, restored mitochondrial membrane potential, and prevented neuronal apoptosis. The mechanism may involve upregulating the expression of proteins such as Bcl-2, SirT-1, OPA1, MFN1, MFN2, and P62, while downregulating the expression of proteins including Bax, cleaved-caspase 3, GRP78, CHOP, p-PERK, p47phox, DRP1, and Beclin-1. This study demonstrates that UC exhibits protective effects against Mncl2-induced neuronal apoptosis, suggesting its potential for development as a neuroprotective functional food.