Resumen de Different lymphocyte markers and cytokine expression in peripheral blood mononuclear cells in children with acute atopic dermatitis
Miguel Blanca Gómez, José Luis Corzo Sierra, Lina Mayorga Mayorga, Antonio Jurado Ortiz, María José Torres Jaén, Cristina Antunez Rodríguez, Rebeca Rodríguez Pena, Luis F. Santamaría Babi
Background: The pathogenesis of atopic dermatitis (AD) is still not completely understood. AD is characterized by the presence of clinical symptoms of both IgE antibody-mediated immediate hypersensitivity and specific T lymphocyte-mediated delayed hypersensitivity.
Objective: To evaluate the immunological mechanisms involved in children with acute AD lesions.
Material and methods: Ten children with acute AD lesions and 10 non-atopic controls were studied. Total IgE was measured by immunoassay. T cell marker expression (CD3, CD4, CD8, cutaneous lymphocyte-associated antigen [CLA]) and cytokine production (interferon [IFN]-?, interleukin [IL]-13) were analyzed in peripheral blood mononuclear cells by flow cytometry.
Results: In children with AD the percentage of CD3+ cells (p = 0.015) increased while that of CD8+ cells (p = 0.023) decreased, with no differences in CLA expression. We found increased IL-13 production in CD3+ cells (p = 0.01) and CD3+CD4+ (p = 0.001) cells with no difference in IFN-?. Total IgE was significantly higher in patients with AD (p = 0.01). Comparison of IL-13 production in CD4+ cells categorized by total IgE level showed that IL-13 production was significantly increased in subjects with a higher IgE level.
Conclusion: Peripheral blood from children with AD showed an increase in IgE levels and a Th2 pattern. There was a correlation between IL-13 production and total IgE levels.
