Different lymphocyte markers and cytokine expression in peripheral blood mononuclear cells in children with acute atopic dermatitis
- Autores: Miguel Blanca Gómez, José Luis Corzo Sierra, Lina Mayorga Mayorga, Antonio Jurado Ortiz, María José Torres Jaén, Cristina Antunez Rodríguez, Rebeca Rodríguez Pena, Luis F. Santamaría Babi
- Localización: Allergologia et immunopathologia: International journal for clinical and investigate allergology and clinical immunology, ISSN-e 1578-1267, ISSN 0301-0546, Vol. 32, Nº. 5, 2004, págs. 252-258
- Idioma: inglés
- Texto completo no disponible (Saber más ...)
-
Resumen
Background: The pathogenesis of atopic dermatitis (AD) is still not completely understood. AD is characterized by the presence of clinical symptoms of both IgE antibody-mediated immediate hypersensitivity and specific T lymphocyte-mediated delayed hypersensitivity.
Objective: To evaluate the immunological mechanisms involved in children with acute AD lesions.
Material and methods: Ten children with acute AD lesions and 10 non-atopic controls were studied. Total IgE was measured by immunoassay. T cell marker expression (CD3, CD4, CD8, cutaneous lymphocyte-associated antigen [CLA]) and cytokine production (interferon [IFN]-?, interleukin [IL]-13) were analyzed in peripheral blood mononuclear cells by flow cytometry.
Results: In children with AD the percentage of CD3+ cells (p = 0.015) increased while that of CD8+ cells (p = 0.023) decreased, with no differences in CLA expression. We found increased IL-13 production in CD3+ cells (p = 0.01) and CD3+CD4+ (p = 0.001) cells with no difference in IFN-?. Total IgE was significantly higher in patients with AD (p = 0.01). Comparison of IL-13 production in CD4+ cells categorized by total IgE level showed that IL-13 production was significantly increased in subjects with a higher IgE level.
Conclusion: Peripheral blood from children with AD showed an increase in IgE levels and a Th2 pattern. There was a correlation between IL-13 production and total IgE levels.
