A simple ethanol wash of the tissue homogenates recovers high-quality genomic DNA from Corchorus species characterized by highly acidic and proteinaceous mucilages

• Avijit Kundu ^[1] ; Debabrata Sarkar ^[1] ; Amit Bhattacharjee ^[1] ; Niladri Topdar ^[1] ; Mohit Kumar Sinha ^[2] ; Bikash Sinha Mahapatra ^[2]
  1. [1] Central Research Institute for Juteand Allied Fibres Division of Crop Improvement Biotechnology Unit
  2. [2] Central Research Institute for Juteand Allied Fibres
• Localización: Electronic Journal of Biotechnology, ISSN-e 0717-3458, Vol. 14, Nº. 1, 2011, págs. 10-11
• Idioma: inglés
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• Resumen

  • A simple miniprep based on early elimination of highly acidic and proteinaceous mucilages through ethanol washing of the tissue homogenates has been developed for the extraction of genomic DNA from mature leaves and seeds of Corchorus spp. As compared to high cetyltrimethylammonium bromide (CTAB)-NaCl DNA extraction followed by ethanol-based removal of remnant mucilages from the DNA pellet, this simple miniprep consistently and reproducibly recovers high amounts of DNA with good spectral qualities at A260/A280 and A260/A230. The purified DNA is efficiently digested by restriction endonucleases, and is suitable for PCR amplification of nuclear microsatellites with expected allele sizes.