Resumen de In vitro trypanocidal activity and chemical constituents of aspilia platyphylla (baker) blake
Rodrigo C. S. Veneziani, Dionéia C. R. de Oliveira, Sérgio de Albuquerque, Wilson R. Cunha
Chloroform and ethanol crude extracts of the underground parts of Aspilia platyphylla (Baker) Blake (Asteraceae - Heliantheae) were submitted to phytochemical investigations and trypanocidal assay. Both extracts were subjected to a partition process with organic solvents. Using a GC analysis method, it was possible to identify the tritepernes β-amyrin, α-amyrin, β-friedelanol, and friedelin, as well as the phytosterol stigmasterol, directly in the n-hexane soluble fraction obtained from the CHCl3 crude extract. Through chromatographic methods and spectroscopic techniques, it was possible to isolate and identify ent-pimara-8(14), 15-dien-19-oic acid; 7-keto-ent-pimara-8(14),15-dien-19-oic acid; 6-acethyl-8-hydroxy-2,2-dimethylchromene; 7β-hydroxy-ent-pimara-8(14),15-dien-19-oic acid; ethyl 6,7-dihydroxycinammate, and rutin in the CH2Cl2 and AcOEt soluble fractions obtained from the CHCl3 and EtOH crude extracts. The CH2Cl2 soluble fractions obtained from the CHCl3 and EtOH crude extracts are active in vitro against the trypomastigote forms of Trypanossoma cruzi (Y strain)
