Arrondissement de Grasse, Francia
Madrid, España
The objective of this work was to study the effect of technical modifications (sponge and filters) in continuous-culture fermenters on the number of protozoa and ruminal fermentation parameters. The effect of the fermentation time was studied as well. Six fermenters fed a 50:50 alfalfa hay:concentrate diet, inoculated with rumen liquor from sheep fed the same diet, were used in two incubation runs of 14 days each. Protozoa numbers of both Entodinia and Holotricha genera and concentrations of VFA and ammonia were determined on days 2, 6, 10 and 14 after inoculation. No effects (P>0.10) of technical modifications were observed either in protozoa numbers or fermentation parameters, although on day 14 protozoa numbers in fermenters provided with sponge and filters were 1.7 and 2.1 times, respectively, higher than those in control fermenters. On the contrary, incubation time affected (P<0.001) all the studied parameters. No treatment x sampling time interaction (P = 0.22 to 0.96) was observed. It is concluded that technical modifications used in this study do not allow for protozoa population maintenance in continuous-culture fermenters.
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