Eficacia de diversas pruebas serológicas para el diagnóstico de la brucelosis porcina
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L. Dieste Perez [2] ; M.J. De Miguel [2] ; C.M. Marín [2] ; M. Barberán [1] ; J.M. Blasco [2] ; P.M. Muñoz [2]
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[1] Universidad de Zaragoza
Universidad de Zaragoza
Zaragoza, España
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[2] CITA
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- Localización: XV Jornadas sobre Producción Animal: 14 y 15 de mayo de 2013, Zaragoza / Jorge Hugo Calvo Lacosta (aut.), Isabel Casasús Pueyo (aut.), Margalida Joy Torrens (aut.), Javier Álvarez Rodríguez (aut.), Luis Varona Aguado (aut.), Begoña Panea Doblado (aut.), Carlos Calvete Margolles (aut.), Joaquín Balcells Teres (aut.), Vol. 2, 2013, ISBN 978-84-695-7684-7, págs. 834-836
- Idioma: español
- Títulos paralelos:
- Performance of several serological tests for diagnosing swine brucellosis
- Enlaces
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Resumen
The S-LPS based serological tests (RB, FC and i-ELISA) used routinely fordiagnosing swine brucellosis do not discriminate the immunological responses due to brucellosis from FPSR due to infections caused by other bacteria like Y. enterocolitica O:9. Gel Diffusion (GD)Counterimmunoelectrophoresis (CIE) and i-ELISA tests using an O-polysaccharide free cytosolic extract obtained from B. abortus manBcore were developed and evaluated to discriminate brucellosis from FPSR. A total of 169 sera from culture positive sows, 423 sera from brucellosis free sows and 185 sera from sows belonging to FPSR affected farms were used for evaluation. The i-ELISA with cytosolic antigen was resulting in very low diagnostic performance. By contrast, GD and CIE with the same cytosolic extract resulted in moderate diagnostic sensitivity (67.5% and 66.9% respectively) but being simultaneously 100% specific in both brucellosis free farms and farms affected by FPSR, offering then a practical alternative to the brucellin skin test to discriminate brucellosis from the FPSR at herd level.
