Activación de la motilidad espermática de verraco post-descongelación
- Autores: José Gómez Fernández, C. Tomás, Emilio Gómez Izquierdo, Eduardo de Mercado de la Peña
- Localización: XV Jornadas sobre Producción Animal: 14 y 15 de mayo de 2013, Zaragoza / Jorge Hugo Calvo Lacosta (aut.), Isabel Casasús Pueyo (aut.), Margalida Joy Torrens (aut.), Javier Álvarez Rodríguez (aut.), Luis Varona Aguado (aut.), Begoña Panea Doblado (aut.), Carlos Calvete Margolles (aut.), Joaquín Balcells Teres (aut.), Vol. 1, 2013, ISBN 978-84-695-7684-7, págs. 437-439
- Idioma: español
- Títulos paralelos:
- Post-thawing activation of boar sperm motility
- Enlaces
-
Resumen
The aim of this study is to determinate if the basic pH and the caffeine, factors that increase the velocity of sperm, are able to recover the latent motility. The effect of the pH in the thawing extender (BTS) to values of 7, 8, 9 and 10, on sperm motility and on sperm viability was evaluated. And for each pH the effect of the use of caffeine (2 mM) or not, on the sperm motility was also evaluated. The results showed that pH above 9, is harmful for the sperm motility and viability. Without caffeine the sperm motility is recovered in more proportion at pH 8 and 9 than control sperm (pH 7 and without caffeine), but without significant difference (P > 0.05). And the combination of basic pH (above 8; pH 9 and 10) and caffeine, was harmful for the sperm motility and viability. However, the thawing extender at pH 8 and with caffeine showed greater motility values than control sperm (percentage of total motile sperm): 42.76 vs 61.3; percentage of progressively motile sperm: 37.48 vs 55.06; P < 0.05). In conclusion, the thawing extender at pH 8 with caffeine (2 mM) is able to recover almost completely the post-thawing latent motility.
