Efecto del hidroxitolueno butilado en la criopreservación espermática de ovinos y caprinos con o sin implantes de melatonina
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[1] Universitat Autònoma de Barcelona
Universitat Autònoma de Barcelona
Barcelona, España
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- Localización: XV Jornadas sobre Producción Animal: 14 y 15 de mayo de 2013, Zaragoza / Jorge Hugo Calvo Lacosta (aut.), Isabel Casasús Pueyo (aut.), Margalida Joy Torrens (aut.), Javier Álvarez Rodríguez (aut.), Luis Varona Aguado (aut.), Begoña Panea Doblado (aut.), Carlos Calvete Margolles (aut.), Joaquim Barcells Teres (aut.), Vol. 1, 2013, ISBN 978-84-695-7684-7, págs. 401-403
- Idioma: español
- Títulos paralelos:
- Effect of butylated hydroxytoluene in conservation of rams and goats semen with or without melatonin implants
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Resumen
The aim of this study was to assess the effect of the inclusion of 5 mM of butylated hydroxytoluene (BHT) outside and inside of the breeding season and the application of melatonin implants on sperm quality in rams and goats. We used 6 Blanca de Rasquera bucks and 8 rams, 4 Aranesa and 4 Xisqueta breeds. The animals were divided into two groups with and without implants of melatonin inserted 60 days before semen collection by artificial vagina. Two ejaculates/day for 2 days/week were collected and mixed according to the experimental group, washed by centrifugation (twice for 10 min at 600g) and re-suspended in freezing medium with or without BHT (5 mM) supplemented with a final concentration of 15% powdered egg yolk and 5% glycerol in a Tris-based medium and refrigerated for 4 hours at 5 °C before freezing. Sperm viability after refrigeration and thawing was determined by eosine-nigrosine stain, no showing differences between treatments in any of the species. Curiously, only on goat sperm chilled in BHT medium, the viability was higher (P<0.01) in animals without that with melatonin implant. However, the time of year had an effect in both species possibly due to the age of the males.
