Evidencias de la ruta de las pentosas fosfato en el espermatozoide ovino

• C. Luna Valiente ^[1] ; E. Serrano Blesa ^[1] ; R. Perez Pé ^[1] ; J. Dominguez Sanz ^[1] ; D. Arruga ^[1] ; A. Casao ^[1] ; J.A. Cebrian Perez ^[1] ; T. Muiño Blanco ^[1]
  1. [1] Universidad de Zaragoza

     Universidad de Zaragoza

     Zaragoza, España

     
• Localización: XV Jornadas sobre Producción Animal: 14 y 15 de mayo de 2013, Zaragoza / Jorge Hugo Calvo Lacosta (aut.), Isabel Casasús Pueyo (aut.), Margalida Joy Torrens (aut.), Javier Álvarez Rodríguez (aut.), Luis Varona Aguado (aut.), Begoña Panea Doblado (aut.), Carlos Calvete Margolles (aut.), Joaquim Barcells Teres (aut.), Vol. 1, 2013, ISBN 978-84-695-7684-7, págs. 410-412
• Idioma: español
• Títulos paralelos:
  • Evidences of the pentose phosphate pathway operating in ram spermatozoa
• Enlaces
  • Texto Completo Libro  1 (pdf)   2 (pdf) 
• Resumen

  • The oxidative stress induced by reactive oxygen species (ROS) is one of the causes of sperm cryopreservation injury. It has been shown that in vitro capacitation results in increased ROS levels, which are dependent of the antioxidant defense system that can use the NADPH generated by the pentose phosphate pathway (PPP). However, the functioning of the PPP has not been demonstrated in ram spermatozoa, although its involvement in sperm capacitation has already been shown in mouse spermatozoa. The aim of this study was to investigate whether the PPP is operative in ram spermatozoa. We have evidenced the presence of G6PDH and 6PGDH in ram spermatozoa by western blotting, indirect immunofluorescence and RT-PCR. Additional assays to evaluate possible changes in the activity of these enzymes during in vitro capacitation are currently in progress.