Receptores de progesterona y estrógeno en el espermatozoide ovino y su correlación con la capacitación

Marta González Arto; Adriana Casao Gascón; Rosaura Pérez Pe; María Teresa Muiño Blanco; José Álvaro Cebrián Pérez; José Alfonso Abecia Martínez

Ayuda

Receptores de progesterona y estrógeno en el espermatozoide ovino y su correlación con la capacitación

M. Gonzalez Arto ^[1] ; A. Casao ^[1] ; R. Perez Pé ^[1] ; T. Muiño Blanco ^[1] ; J.A. Cebrian Perez ^[1] ; José Alfonso Abecia Martínez
1. [1] Universidad de Zaragoza
  
  Universidad de Zaragoza
  
  Zaragoza, España
Localización: XV Jornadas sobre Producción Animal: 14 y 15 de mayo de 2013, Zaragoza / Jorge Hugo Calvo Lacosta (aut.), Isabel Casasús Pueyo (aut.), Margalida Joy Torrens (aut.), Javier Álvarez Rodríguez (aut.), Luis Varona Aguado (aut.), Begoña Panea Doblado (aut.), Carlos Calvete Margolles (aut.), Joaquín Balcells Teres (aut.), Vol. 1, 2013, ISBN 978-84-695-7684-7, págs. 413-415
Idioma: español
Títulos paralelos:
- Progesterone receptor and estrogen receptor in ram spermatozoa and their correlation with the capacitation state
Enlaces
- Texto Completo Libro 1 (pdf) 2 (pdf)
Resumen
- Progesterone and estradiol play an important role in sperm function, especially in sperm capacitation, and these actions are likely receptor mediated. We have previously shown the presence of estrogen receptor (ER) in ram spermatozoa, with three different inmunotypes. However, there are no reports on progesterone receptor (PR) in this species. Given that capacitation and the acrosome reaction involve sperm membrane changes and molecular redistribution, it can be hypothesized that the receptor distribution of these hormones can be modified. Thus, in this study we investigated the presence of progesterone receptor (PR) in ram spermatozoa, and analyzed the distribution of both PR and ER in different physiological states associated with ram sperm capacitation. Using immuno fluoresce techniques, we identified for the first time PR in ram spermatozoa, located in the equatorial region. Moreover, our results showed that PR distribution remained independent of the samples’ capacitation state. However, ER distribution varied depending on the capacitation state. A high positive correlation between the presence of ER in the acrosomal apical zone and the rate of non-capacitated spermatozoa (r=0.848; P<0.001) was found. These results suggest that ER could be involved in ram sperm capacitation.