Resumen de Efectos del ácido hialurónico, en distintas concentraciones y pesos moleculares, sobre la supervivencia de los embriones ovinos producidos in vitro

Vahid Ghaffarilaleh, F. Ghafari, María Teresa Paramio Nieto, A. A. Fouladi-Nashta

• Hyaluronic Acid (HA), a component of extracellular matrix in mammalian tissues including that of the reproductive system, has been shown to support embryo survival. HA is produced in various sizes with distinct physiological functions. In vitro produced 4 + cell stage embryos were cultured in serum free synthetic oviduct fluid with different concentrations of different sizes HA without (control) or supplemented with three increasing concentrations of healon (molecular weight 5 × 106 Da, 0.125, 0.25 and 0.5 mg/ml), hyalovet (molecular weight 500 - 750 × 103 Da, 0.25, 0.50 and 1 mg/ml) and hyal2 (molecular weight 55 × 103 Da, 75, 150 and 300 IU/ml). Blastocysts from each treatment/repeat were vitrified in open pulled straws. Thereafter, thawed embryos cultured for an extra period of 48 h to analyse their survival rate. SPSS version 20 software was used for analyzing the data with generalized linear model. HA in large size numerically increased survival rates 48 h after culture in serum free media (38±17.1, 63±17.1, 83±15.2 and 58±14.2; P>0.05), but medium size (57±6.45, 71±6.45, 75±10.83 and 85±5.00, P 0.05), and small size (38±13.5, 92±07.04, 43±13.2 and 41±11.9, P 0.05) improved the survival rate. In conclusion HA has ability to improve the viability of frozen blastocysts after freezing.