Efectos del ácido hialurónico, en distintas concentraciones y pesos moleculares, sobre la supervivencia de los embriones ovinos producidos in vitro

• V. Ghaffarilaleh ^[1] ; F. Ghafari ^[3] ; M.T. Paramio ^[2] ; A.A. Fouladi-Nashta ^[1]
  1. [1] Royal Veterinary College

     Royal Veterinary College

     Reino Unido

     
  2. [2] Universitat Autònoma de Barcelona

     Universitat Autònoma de Barcelona

     Barcelona, España

     
  3. [3] Walsgrave University Hospital

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• Localización: XV Jornadas sobre Producción Animal: 14 y 15 de mayo de 2013, Zaragoza / Jorge Hugo Calvo Lacosta (aut.), Isabel Casasús Pueyo (aut.), Margalida Joy Torrens (aut.), Javier Álvarez Rodríguez (aut.), Luis Varona Aguado (aut.), Begoña Panea Doblado (aut.), Carlos Calvete Margolles (aut.), Joaquín Balcells Teres (aut.), Vol. 1, 2013, ISBN 978-84-695-7684-7, págs. 365-367
• Idioma: español
• Títulos paralelos:
  • Effect of different concentrations and molecular weith of hyaluronic acid on survival of in vitro sheep embryos after cryopreservation
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  • Texto Completo Libro  1 (pdf)   2 (pdf) 
• Resumen

  • Hyaluronic Acid (HA), a component of extracellular matrix in mammalian tissues including that of the reproductive system, has been shown to support embryo survival. HA is produced in various sizes with distinct physiological functions. In vitro produced 4 + cell stage embryos were cultured in serum free synthetic oviduct fluid with different concentrations of different sizes HA without (control) or supplemented with three increasing concentrations of healon (molecular weight 5 × 106 Da, 0.125, 0.25 and 0.5 mg/ml), hyalovet (molecular weight 500 - 750 × 103 Da, 0.25, 0.50 and 1 mg/ml) and hyal2 (molecular weight 55 × 103 Da, 75, 150 and 300 IU/ml). Blastocysts from each treatment/repeat were vitrified in open pulled straws. Thereafter, thawed embryos cultured for an extra period of 48 h to analyse their survival rate. SPSS version 20 software was used for analyzing the data with generalized linear model. HA in large size numerically increased survival rates 48 h after culture in serum free media (38±17.1, 63±17.1, 83±15.2 and 58±14.2; P>0.05), but medium size (57±6.45, 71±6.45, 75±10.83 and 85±5.00, P 0.05), and small size (38±13.5, 92±07.04, 43±13.2 and 41±11.9, P 0.05) improved the survival rate. In conclusion HA has ability to improve the viability of frozen blastocysts after freezing.