Colombia
This study was conducted to evaluate the effects of three exogenous fibrolytic enzymes (cellulase from Trichoderma longibrachiatum (CEL), xylanase from ruminal microorganisms (XYL) and a 1:1 mixture of both enzymes (MIX)) on the in vitro ruminal fermentation of three tropical forages (Pennisetum clandestinum, Dichanthium aristatum Benth and Acacia mangium). Forages were treated with enzymes for 24 h before in vitro incubation with ruminal fluid for 24 h. Both CEL and MIX treatments increased (P<0.05) dry matter and fiber disappearance for P. clandestinum and D. aristatum Benth, but had no effects (P>0.05) with A. mangium. In addition, CEL increased (P<0.05) propionate and butyrate production for all forages. In contrast, no effects (P>0.05) of XYL were detected for any forage. The results indicate that the treatment of tropical forages with CEL and MIX can stimulate their in vitro ruminal fermentation, but the xylanase used in this study did not produce any positive effect.
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