Resumen de Development of nanoparticle complexes as liquid biopsy candidates for circulating tumor cell detection
Gabriela Caldero Linnhoff, Carlos Rodríguez Abreu, Eliana Rosales, Genoveva Morral Ruíz, Pedro Melgar Lesmes, Stefanie Leitner, Conxita Solans, María José García Celma
Early detection of diseases is known to improve significantly the prognosis of many diseases. Liquid biopsy tests are emerging as an interesting approach, as they make diagnosis results available in a fast and simple way by just using biological fluids obtained in a minimally invasive manner. Certain solid tumour types, such as breast cancer, non-small cell lung cancer, colorectal cancer or prostate cancer, may be diagnosed by detecting circulating tumour cells (CTC) [1]. It is worth mentioning that the folate receptor is often highly expressed in these solid tumour types and may be considered as a biomarker. In this context, the use of nanoparticles may enhance the performance of liquid biopsy devices, through the specific recognition of disease biomarkers and enabling their detection in tiny volumes of body fluids.
In the present research work, the potential of ethylcellulose nanoparticles as liquid biopsy nanodiagnostic tools has been preliminarily explored by using affordable materials and smooth preparation methods. For this purpose, the formation of positively charged ethylcellulose nanoparticles has been investigated using a low-energy emulsification method. Further, the ethylcellulose nanoparticles have been functionalized with gold nanoparticles or folate exploiting supramolecular interactions. Thehaemocompatibility of the latter complexes has been assessed as a key factor in diagnostic tests involving the analysis of blood samples.
