Development of nanoparticle complexes as liquid biopsy candidates for circulating tumor cell detection
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Gabriela Caldero Linnhoff [1] [3] [2] ; Carlos Rodríguez Abreu [3] [2] ; Rosales E [3] ; Genoveva Morral Ruíz [1] ; Pedro Melgar Lesmes [1] ; Stefanie Leitner [3] [2] ; Solans C [3] [2] ; María José García Celma [1] [2]
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[1] Universitat de Barcelona
Universitat de Barcelona
Barcelona, España
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[2] Centro de Investigación Biomédica en Red sobre Bioingeniería, Biomateriales y Nanomedicina
Centro de Investigación Biomédica en Red sobre Bioingeniería, Biomateriales y Nanomedicina
Madrid, España
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[3] Institute of Advanced Chemistry of Catalonia (IQAC), Spanish National Research Council (CSIC), Jordi Girona 18-26, 08034, Barcelona, Spain.
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- Localización: RESCIFAR Revista Española de Ciencias Farmacéuticas, ISSN-e 2660-6356, Vol. 2, Nº. 2, 2021 (Ejemplar dedicado a: XV CONGRESO DE LA SOCIEDAD ESPAÑOLA DE FARMACIA INDUSTRIA Y GALÉNICA), págs. 127-129
- Idioma: inglés
- Enlaces
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Resumen
Early detection of diseases is known to improve significantly the prognosis of many diseases. Liquid biopsy tests are emerging as an interesting approach, as they make diagnosis results available in a fast and simple way by just using biological fluids obtained in a minimally invasive manner. Certain solid tumour types, such as breast cancer, non-small cell lung cancer, colorectal cancer or prostate cancer, may be diagnosed by detecting circulating tumour cells (CTC) [1]. It is worth mentioning that the folate receptor is often highly expressed in these solid tumour types and may be considered as a biomarker. In this context, the use of nanoparticles may enhance the performance of liquid biopsy devices, through the specific recognition of disease biomarkers and enabling their detection in tiny volumes of body fluids.
In the present research work, the potential of ethylcellulose nanoparticles as liquid biopsy nanodiagnostic tools has been preliminarily explored by using affordable materials and smooth preparation methods. For this purpose, the formation of positively charged ethylcellulose nanoparticles has been investigated using a low-energy emulsification method. Further, the ethylcellulose nanoparticles have been functionalized with gold nanoparticles or folate exploiting supramolecular interactions. Thehaemocompatibility of the latter complexes has been assessed as a key factor in diagnostic tests involving the analysis of blood samples.
