Implementation of an At-Home, First-Semester Biochemistry Lab Course: A Module Based on Banana Tyrosinase
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Callie Barton [1] ; Katherine Clohan Gasaway [1] ; Rejaul Islam [1] ; Tarfi Aziz [1] ; Jessica Krewall [1] ; Devon Punthrangkul [1] ; Kyle R. Willian [1]
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[1] Auburn University
Auburn University
Estados Unidos
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- Localización: Journal of chemical education, ISSN 0021-9584, Vol. 99, Nº 4, 2022, págs. 1571-1578
- Idioma: inglés
- Texto completo no disponible (Saber más ...)
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Resumen
This paper describes the development of a fully remote upper-class biochemistry lab course. The sudden change to online teaching in the middle of spring semester 2020 had a primarily negative impact on laboratory teaching. These effects were mitigated because the students had done many of the basic hands-on procedures before the switch. A true “at-home” biochemistry lab module was implemented in the fall semester of 2020 to ensure students could have a hands-on lab experience in a remote setting despite the remaining COVID-19 restrictions placed upon universities. The module covered several fundamental concepts and techniques found in a first semester biochemistry lab sequence: extraction and purification of a protein from a sample, and further analysis of the protein. Tyrosinase was isolated and purified from a banana extract followed by kinetic analysis of the enzyme. A key component to the module is an LED light board that, in combination with a cell-phone app, made a simple at-home colorimeter. The module was implemented in three sections of a first semester biochemistry lab course (81 students total) in the fall of 2020, and components of it have been used periodically since. Some of the procedures are now being implemented into normal in-lab sessions. An assessment in terms of a student survey showed that most of the students were able to adapt to this format and felt that their learning was not impeded.
