Gene variant space for biosensor-based dynamic regulation

• María Camarena ^[1] ; Yadira Boada ^[1] ; Jesús Picó ^[1] ; Pablo Carbonell
  1. [1] Universidad Politécnica de Valencia

     Universidad Politécnica de Valencia

     Valencia, España

     
• Localización: XLII Jornadas de Automática: libro de actas, Castellón, 1 a 3 de septiembre de 2021, 2021, ISBN 978-84-9749-804-3, págs. 485-491
• Idioma: inglés
• Enlaces
  • Texto completo
• Resumen

  • Inside a cell, protein, production and biosensor pathways can be genetically engineered within a dynamic regulation architecture that provides robustness to cell factories. Here we investigated how the selection of gene variants and their associated expression efficiency and kinetic parameters can lead to a wide diversity of dynamic responses in terms of protein or metabolite production. Results show that there is a trade-off between gene expression efficiency and pathway performance, and it can be eventually related to the evolutionary fingerprint of each gene variant. Therefore, the organism source of gene variants is a factor that needs to be considered in the design of dynamic regulation for genetic circuits.