The beneficial effects of physical activity and weight loss on human colorectal carcinoma cell lines

• Rita Polito ^[1] ; Alessia Scarinci ^[5] ; Antonio Ambrosi ^[1] ; Nicola Tartaglia ^[1] ; Domenico Tafuri ^[6] ; Marcellino Monda ^[2] ; Antonietta Messina ^[2] ; Fabiano Cimmino ^[3] ; Angela Catapano ; Francesco Sessa ^[1] ; Girolamo di Maio ^[2] ; Vincenzo Cristian Francavilla ^[4] ; Giovanni Messina ^[1] ; Vincenzo Monda
  1. [1] University of Foggia

     University of Foggia

     Foggia, Italia

     
  2. [2] Second University of Naples

     Second University of Naples

     Caserta, Italia

     
  3. [3] University of Naples Federico II

     University of Naples Federico II

     Nápoles, Italia

     
  4. [4] Università degli Studi di Enna Kore

     Università degli Studi di Enna Kore

     Enna, Italia

     
  5. [5] University of Bari
  6. [6] University of Naples "Parthenope"

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• Localización: Journal of Human Sport and Exercise: JHSE, ISSN-e 1988-5202, Vol. 15, Nº. Extra 2, 2020 (Ejemplar dedicado a: Winter Conferences of Sports Science), págs. 252-260
• Idioma: inglés
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• Resumen

  • Recent studies have demonstrated that obesity is a significant risk factor for the development of several malignancies such as cancer. Colorectal cancer is among the most common cancers worldwide and is strong linked to obesity. A healthy lifestyle, characterized by hypocaloric diet and physical activity, is important to reduce a chronic inflammation, oxidative stress and metabolic disorders typical of obesity (Messina et al, 2018; Messina et al, 2017; Messina et al, 2015). It is well known that the chronic inflammation state and oxidative stress are responsible for the aging and development of many diseases, such as cancer. Dysregulation of cytokine’s secretion probably participates in the establishment of cancer in obese patients. The aim of this study is to analyse the effects of sera from obese patients subjected to a physical activity program before and after weight loss on cell viability, apoptosis and oxidative stress in HCT116 carcinoma cell line treated for 24, 48 and 72 hours through MTT test. We analysed the expression of cytokines in HCT116 cells. We found that sera from obese after physical activity intervention compared to treatment with sera from obese patients before physical activity intervention reduce the survival rate of HCT116 cells through induction of apoptosis and oxidative stress. Finally, we found a reduction of mRNA levels corresponding to the pro-inflammatory IL-6 and IL-8 cytokines together with an increase of the anti-inflammatory IL-10 cytokine. We can conclude that the physical activity has numerous beneficial effects also in colorectal cancer cell, indeed the physical activity and weight loss in obese subjects have an inhibitory and anti-inflammatory effects in a short period on carcinoma cell line.