Metabolic characterization of two different non-alcoholic fatty liver disease pre-clinical mouse models

• Rocío Gallego-Durán ^[1] ; Leticia Álvarez-Amor ^[2] ; Antonio Gil-Gómez ^[1] ; Ángela Rojas ^[1] ; Rocío Muñoz-Hernández ^[1] ; Antonio Cádernas-García ^[2] ; Douglas Maya-Miles ^[1] ; Rocío Montero-Vallejo ^[1] ; Sheila Gato ^[1] ; Yolanda Sánchez Torrijos ^[1] ; Javier Ampuero ^[1] ; Francisco Martín ^[2] ; Manuel Romero-Gómez ^[1]
  1. [1] Hospital Universitario Virgen del Rocío

     Hospital Universitario Virgen del Rocío

     Sevilla, España

     
  2. [2] Centro Andaluz de Biología Molecular y Medicina Regenerativa

     Centro Andaluz de Biología Molecular y Medicina Regenerativa

     Sevilla, España

     
• Localización: Revista Española de Enfermedades Digestivas, ISSN-e 2340-4167, ISSN 1130-0108, Vol. 111, Nº. 4, 2019, págs. 301-307
• Idioma: inglés
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• Resumen

  • Introduction: non-alcoholic fatty liver disease is one of the most prevalent liver disorders in the developed world. Currently, there is no approved pharmacological therapy except for lifestyle intervention. Therefore, there is a need to increase the knowledge of preclinical models in order to boost novel discoveries that could lead to a better therapeutic management. Material and methods: this study characterized the effects of two different diets, a long-term high-fat high-fructose diet (HF-HFD) and a choline-deficient, methionine supplemented high-fat diet (CDA-HFD) in C57BL/6J mice for 52 weeks or 16 weeks, respectively. Body weight, lipid and hepatic profile were analyzed and liver histology was subsequently evaluated. Results: HF-HFD animals had an increased body weight and total cholesterol levels, whereas the opposite occurred in CDA-HFD. Both HF-HFD and CDA-HFD animals had higher ALT and AST levels. With regard to histology findings, HF-HFD and CDA-HFD diets induced an increased collagen deposit and intrahepatic steatosis accumulation. Conclusion: in conclusion, the comparison of these models aided in the selection of a long-term, more physiological model for physiopathology studies or a more rapid NASH model for novel molecule testing.