Vanillin Production in Pseudomonas: Whole-Genome Sequencing of Pseudomonas sp. Strain 9.1 and Reannotation of Pseudomonas putida CalA as a Vanillin Reductase

• Javier García-Hidalgo ^[1] ; Daniel P. Brink ^[1] ; Krithika Ravi ^[1] ; Catherine J. Paul ^[1] ; Gunnar Lidén ^[1] ; Marie F. Gorwa-Grauslund ^[1]
  1. [1] Lund University

     Lund University

     Suecia

     
• Localización: Applied and Environmental Microbiology, ISSN 0099-2240, Vol. 86, Nº 6, 2020
• Idioma: inglés
• Enlaces
  • Texto completo
• Resumen

  • Valorization of lignocellulose (nonedible plant matter) is of key interest for the sustainable production of chemicals from renewable resources. Lignin, one of the main constituents of lignocellulose, is a heterogeneous aromatic biopolymer that can be chemically depolymerized into a heterogeneous mixture of aromatic building blocks; those can be further converted by certain microbes into value-added aromatic chemicals, e.g., the flavoring agent vanillin. We previously isolated a Pseudomonas sp. strain with the (for the genus) unusual trait of vanillyl alcohol production during growth on vanillin. Whole-genome sequencing of the isolate led to the identification of a vanillin reductase candidate gene whose deletion in a recombinant vanillin-accumulating P. putida strain almost completely alleviated the undesired vanillyl alcohol by-product yield. These results represent an important step toward biotechnological production of vanillin from lignin using bacterial cell factories.