Identification of Proteins Using Four Methods of Matrix-Assisted Laser Desorption Ionization Time-of-Flight Mass Spectrometry

• Kestutis G. Bendinskas ^[1] ; Ashley J. Canning ^[1] ; Ashlee E. Smith ^[1]
  1. [1] State University of New York

     State University of New York

     City of Albany, Estados Unidos

     
• Localización: Journal of chemical education, ISSN 0021-9584, Vol. 96, Nº 11, 2019, págs. 2600-2605
• Idioma: inglés
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• Resumen

  • An upper-division undergraduate- or graduate-level laboratory experiment, challenging students to identify bovine serum albumin, equine myoglobin, bovine calmodulin, and bovine cytochrome c, uses four methods of matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS): linear positive, in-source decay, peptide mass fingerprint, and postsource decay. Individual hands-on experience with each method is provided in a three week experiment. The importance of sequencing proteins/peptides when identifying proteomic targets using MALDI-TOF MS is emphasized.