Kinetics and Identities of Extracellular Peptidases in Subsurface Sediments of the White Oak River Estuary, North Carolina

• Andrew D. Steen ^[2] ; Richard T. Kevorkian ^[3] ; Jordan T. Bird ^[3] ; Nina Dombrowski ^[4] ; Brett J. Baker ^[4] ; Shane M. Hagen ^[5] ; Katherine H. Mulligan ^[6] ; Jenna M. Schmidt ^[5] ; Austen T. Webber ^[3] ; Taylor M. Royalty ^[5] ; Marc J. Alperin ^[1]
  1. [1] University of North Carolina at Chapel Hill

     University of North Carolina at Chapel Hill

     Township of Chapel Hill, Estados Unidos

     
  2. [2] a Department of Microbiology, University of Tennessee, Knoxville, Knoxville, Tennessee, USA; b Department of Earth and Planetary Sciences, University of Tennessee, Knoxville, Knoxville, Tennessee, USA
  3. [3] a Department of Microbiology, University of Tennessee, Knoxville, Knoxville, Tennessee, USA
  4. [4] c Department of Marine Science, University of Texas—Austin, Marine Science Institute, Port Aransas, Texas, USA
  5. [5] b Department of Earth and Planetary Sciences, University of Tennessee, Knoxville, Knoxville, Tennessee, USA
  6. [6] b Department of Earth and Planetary Sciences, University of Tennessee, Knoxville, Knoxville, Tennessee, USA; d Department of Biology, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina, USA

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• Localización: Applied and Environmental Microbiology, ISSN 0099-2240, Vol. 85, Nº 19, 2019
• Idioma: inglés
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• Resumen

  • Burial of organic carbon in marine and estuarine sediments represents a long-term sink for atmospheric carbon dioxide. Globally, ∼40% of organic carbon burial occurs in anoxic estuaries and deltaic systems. However, the ultimate controls on the amount of organic matter that is buried in sediments, versus oxidized into CO2, are poorly constrained. In this study, we used a combination of enzyme assays and metagenomic analysis to identify how subsurface microbial communities catalyze the first step of proteinaceous organic carbon degradation. Our results show that microbial communities in deeper sediments are adapted to access molecules characteristic of degraded organic matter, suggesting that those heterotrophs are adapted to life in the subsurface.