Changes in the Ileal, but Not Fecal, Microbiome in Response to Increased Dietary Protein Level and Enterotoxigenic Escherichia coli Exposure in Pigs

• Jolinda Pollock ^[2] ; Michael R. Hutchings ^[3] ; Kate E. K. Hutchings ^[3] ; David L. Gally ^[1] ; Jos G. M. Houdijk ^[3]
  1. [1] University of Edinburgh

     University of Edinburgh

     Reino Unido

     
  2. [2] a Animal and Veterinary Sciences, Scotland’s Rural College (SRUC), Edinburgh, United Kingdom; b The Roslin Institute and Royal (Dick) School of Veterinary Studies, University of Edinburgh, Edinburgh, United Kingdom
  3. [3] a Animal and Veterinary Sciences, Scotland’s Rural College (SRUC), Edinburgh, United Kingdom

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• Localización: Applied and Environmental Microbiology, ISSN 0099-2240, Vol. 85, Nº 19, 2019
• Idioma: inglés
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  • Gut bacterial communities have been shown to play a key role in pig health and development and are strongly influenced by host diet, but studies highlighting the complex interactions between nutrition, gut infections and the microbiome tend to focus on bacterial populations in the feces and not other important gut locations. We found that alteration of dietary protein level and exposure to a pathogenic microorganism, enterotoxigenic Escherichia coli (ETEC), changed bacterial populations in the distal small intestine (i.e., the ileum). We found that the most profound changes occurred in pigs fed a high-protein diet in combination with exposure to ETEC, showing a clear interaction between dietary composition and exposure to a key pathogen. These changes were not observed in the fecal samples, revealing the importance of studying biologically pertinent sites in the gut, and so the data will help to inform the development of alternative management strategies for enteric disorders.