Reduction of Escherichia coliO157: H7 in Biofilms Using Bacteriophage BPECO 19

• Autores: Mohammad Sadekuzzaman, Sungdae Yang, M. Furkanur Rahaman Mizan, Sang-Do Ha
• Localización: Journal of food science, ISSN 0022-1147, Vol. 82, Nº 6, 2017, págs. 1433-1442
• Idioma: inglés
• Texto completo no disponible (Saber más ...)
• Resumen

  • Biofilm formation is a growing concern in the food industry. Escherichia coliO157:H7 is one of the most important foodborne pathogens that can persists in food and food‐related environments and subsequently produce biofilms. The efficacy of bacteriophage BPECO 19 was evaluated against three E. coliO157:H7 strains in biofilms. Biofilms of the three E. coliO157:H7 strains were grown on abiotic (stainless steel, rubber, and minimum biofilm eradication concentration [MBECTM] device) and biotic (lettuce) surfaces at different temperatures. The effectiveness of bacteriophage BPECO 19 in reducing preformed biofilms on these surfaces was further evaluated by treating the surfaces with a phage suspension (108PFU/mL) for 2 h. The results indicated that the phage treatment significantly reduced (P< 0.05) the number of adhered cells in all the surfaces. Following phage treatment, the viability of adhered cells was reduced by ≥3 log CFU/cm2, 2.4 log CFU/cm2, and 3.1 log CFU/peg in biofilms grown on stainless steel, rubber, and the MBECTMdevice, respectively. Likewise, the phage treatment reduced cell viability by ≥2 log CFU/cm2in biofilms grown on lettuce. Overall, these results suggested that bacteriophages such as BPECO 19 could be effective in reducing the viability of biofilm‐adhered cells. Our results suggested that bacteriophages such as BPECO 19 could be effective in reducing the viability of biofilm‐adhered cells. Therefore, it could serve as a practical method to control the growth of E. coliO157:H7 in biofilms. In fact, bacteriophages could be part of an ideal, eco‐friendly biocontrol intervention strategy to control pathogens in biofilms.