Breeding of Pleurotus florida (oyster mushroom) for phenotypic pigmentation and high yield potential

• Autores: Jatinder Kaur, Harpreet S Sodhi, Shammi Kapoor
• Localización: Journal of the science of food and agriculture, ISSN 0022-5142, Vol. 88, Nº 15, 2008, págs. 2676-2681
• Idioma: inglés
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• Resumen

  • BACKGROUND: Cross-hybridisation is a technique for exchange of genetic material between two compatible nuclei to develop a recombinant genome with a probable expression for a desirable trait. This technique as an example of classical genetics has been applied in a heterothallic bifactorial/tetrapolar fungus Pleurotus florida. It has worked successfully during this study in a small number of experiments. RESULTS: Fruit bodies from the Pleurotus florida PAU-5 were allowed to shed their basidiospores on filter paper under aseptic conditions. Forty-nine monokaryons were isolated from three spore prints, namely Ja, Jb and K. Three hundred and fifty-six crosses were laid to result in five compatible reactions (PFJ4, PFJ9, PFJ11, PFJ13 and PFJ14). The fruit bodies of the hybrid dikaryon PFJ4 were found to show grey pigmentation. The hybrid dikaryons PFJ11 and PFJ14 grew faster in wheat straw substrate to take 39 and 41 days, respectively, for complete mycelial impregnation as compared to the parent, PAU-5 (48 days). The dikaryon PFJ11 out-yielded the parent by giving 34.2% biological efficiency compared to 29.8% for the parent. CONCLUSION: Through cross-hybridisation various changes at the genetic level are possible, showing altered phenotypic expression of the characters, such as change in fruiting efficiency and variability in fruit body characteristics (e.g., pileus shape and pigmentation). This technique can also be applied to other crops to improve their yield potential and bring about desirable phenotypic changes. Copyright © 2008 Society of Chemical Industry