Sporadic incidence of factor XI deficiency in Holstein cattle

• Autores: Jindřich Čítek, Václav Řehout, Lenka Hanusová, Petra Vrabcová
• Localización: Journal of the science of food and agriculture, ISSN 0022-5142, Vol. 88, Nº 12, 2008, págs. 2069-2072
• Idioma: inglés
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• Resumen

  • BACKGROUND: Factor XI (FXI) is a plasma serine protease involved in the early activation of the blood coagulation cascade. A survey of Holstein cattle, and Czech Simmental cattle crossed with Holstein, was done to determine whether animals heterozygous for FXI deficiency were present. Mutations in exon 12 and in exon 9 were analysed. RESULTS: The method used was based on the polymerase chain reaction and subsequent distinction of the length of fragments. In the panel analysed (279 Holsteins and 30 Simmentals), one heterozygous carrier of the recessive allele in exon 12 (a German Holstein cow) was found. Some authors have found that the frequency is high in some breeding lines and low in others. Evidently the lines in our analysis are part of the group with a low frequency. In exon 9, all the animals (100 Holsteins and 52 Simmentals), were homozygous wild type. To date, the mutation in exon 9 has been found only in Japanese black cattle, and its breed-specific occurrence was therefore not disconfirmed by our assay. CONCLUSION: Our results revealed a low occurrence of F11 mutant alleles. The DNA-based test determines all genotypes regardless of phenotype and FXI activity. This is important as veterinarians need a reliable method. Of course, the testing of numerous sires by molecular methods is not necessary from the breeders' point of view, but in breeding lines where testing is deemed appropriate heterozygous animals could be easily and surely detected. Copyright © 2008 Society of Chemical Industry