Tenderizing Effect of High Hydrostatic Pressure on Bovine Intramuscular Connective Tissue

• Autores: S. Ichinoseki, T. Nishiumi, A. Suzuki
• Localización: Journal of food science, ISSN 0022-1147, Vol. 71, Nº 6, 2006
• Idioma: inglés
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• Resumen

  • The effect of high-pressure treatment on bovine intramuscular connective tissue was investigated under various pressures (0.1 to 500 MPa) at 8 °C for 5 min. Shear force value, heat solubility of collagen, and structural changes of pressurized intramuscular connective tissues were evaluated. High hydrostatic pressure tenderized bovine intramuscular connective tissue as well as meat. The heat solubility of collagen increased (P < 0.05) with high pressure, which indicates a reduction of collagen thermal stability. However, high pressure could not degrade collagen molecules by sodium dodecyl sulfate–polyacrylamide gel electrophoresis analysis. Collagen-derived peptide analysis also suggested that high pressure could not degrade the molecular structure of collagen, but could dissociate the collagen fibers or fibrils into fibrils or molecules. Histological study showed that high pressure induced structural weakening of intramuscular connective tissue, especially perimysium.