Demonstrating Basic Properties of Spectroscopy Using a Self-Constructed Combined Fluorimeter and UV-Photometer

• Eivind V. Kvittingen ^[2] ; Lise Kvittingen ^[1] ; Thor Bernt Melø ^[1] ; Birte Johanne Sjursnes ^[3] ; Richard Verley ^[2]
  1. [1] Norwegian University of Science and Technology

     Norwegian University of Science and Technology

     Noruega

     
  2. [2] Overlege Kindts, Norway
  3. [3] Østfold University College, Norway

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• Localización: Journal of chemical education, ISSN 0021-9584, Vol. 94, Nº 10, 2017, págs. 1486-1491
• Idioma: inglés
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• Dialnet Métricas: 1 Cita
• Resumen

  • This article describes a combined UV-photometer and fluorimeter constructed from 3 LEDs and a few wires, all held in place with Lego bricks. The instrument has a flexible design. In its simplest version, two UV-LEDs (355 nm) are used as light source and to detect absorption, and a third LED, in the visible spectrum (e.g., 525 nm), is used to detect fluorescence. Various experiments are described: fluorescence as a function of concentration and light source intensity; quantitative measurements of quinine in tonic water, using absorption and fluorescence measurements, with results in line with those obtained on scientific instruments; a demonstration of the inner filter effect reducing fluorescence at higher concentrations; investigation of the effect of drinking tonic water on the fluorescence of quinine in urine; temperature effects on fluorescence; chloride-ion quenching of fluorescence with result comparable to those previously reported; and, finally, a demonstration of phosphorescence.