Enzyme Kinetics Experiment with the Multienzyme Complex Viscozyme L and Two Substrates for the Accurate Determination of Michaelian Parameters

• Nelson Pérez Guerra ^[1]
  1. [1] Universidade de Vigo

     Universidade de Vigo

     Vigo, España

     
• Localización: Journal of chemical education, ISSN 0021-9584, Vol. 94, Nº 6, 2017, págs. 795-799
• Idioma: inglés
• Texto completo no disponible (Saber más ...)
• Resumen

  • A laboratory experiment in which students study the kinetics of the Viscozyme-L-catalyzed hydrolysis of cellulose and starch comparatively was designed for an upper-division biochemistry laboratory. The main objective of this experiment was to provide an opportunity to perform enhanced enzyme kinetics data analysis using appropriate informatics and statistical tools. By fitting the Michaelis–Menten, Lineweaver–Burk, Eadie–Hofstee, and Hanes equations to the experimental data, students explore the strengths and weaknesses of each plot and determine whether the two Viscozyme-L-catalyzed reactions obey Michaelis–Menten kinetics. Comparing the values of the Michaelian constants (vmax and KM) and the efficiencies obtained for each enzyme-catalyzed reaction allows advanced undergraduates to determine if the differences between these parameters are statistically significant.