Extraction and Antibacterial Properties of Thyme Leaf Extracts: Authentic Practice of Green Chemistry

• Sean C. Purcell ^[1] ; Prithvi Pande ^[1] ; Yingxin Lin ^[1] ; Ernesto J. Rivera ^[1] ; Latisha Paw U ^[1] ; Luisa M. Smallwood ^[1] ; Geri A. Kerstiens ^[1] ; Laura B. Armstrong ^[1] ; MaryAnn T. Robak ^[1] ; Anne M. Baranger ^[1] ; Michelle C. Douskey ^[1]
  1. [1] University of California, United States
• Localización: Journal of chemical education, ISSN 0021-9584, Vol. 93, Nº 8, 2016, págs. 1422-1427
• Idioma: inglés
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• Resumen

  • In this undergraduate analytical chemistry experiment, students quantitatively assess the antibacterial activity of essential oils found in thyme leaves (Thymus vulgaris) in an authentic, research-like environment. This multiweek experiment aims to instill green chemistry principles as intrinsic to chemical problem solving. Students progress through various techniques including extraction, chromatography (TLC and HPLC), culturing bacteria, and disk diffusion via a process of guided exploration that emphasizes green experimental design. Approximately 600 undergraduate students carried out the experiment and self-reported substantial learning gains.