Biocatalytic Lactone Generation in Genetically Engineered Escherichia coli and Identification of Products by Gas Chromatography-Mass Spectroscopy
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[1] University of Florida
University of Florida
Estados Unidos
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[2] University of South Florida
University of South Florida
Estados Unidos
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- Localización: Journal of chemical education, ISSN 0021-9584, Vol. 78, Nº 11, 2001, pág. 1533
- Idioma: inglés
- Texto completo no disponible (Saber más ...)
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Resumen
Genetically altered Escherichia coli are used as biocatalysts to produce optically pure lactones from a variety of cyclic ketones as a biotechnology experiment for a biochemistry laboratory. The genetically engineered E. coli bacteria express large amounts of the enzyme cyclohexanone monooxygenase and are therefor capable of converting a variety of ketones into optically pure lactones. Separation by organic extraction and analysis by thin layer chromatography and gas chromatography-mass spectroscopy allows for the direct identification of products. Yield calculations and evaluation of the cost effectiveness of various substrates give students an opportunity to make recommendations and model industrial decision-making. Evaluation of the synthetic process for its environmental impact allows students to consider problems of cost versus environmental concerns. Use of bacterial biocatalysts offers chemistry students an opportunity to work with microorganisms and directly see the utility of genetically altered bacteria for synthetic chemistry.
