Resumen de Studying Cooperative Ligand Binding in the Undergraduate Biochemistry Laboratory: Oxygen–Hemoglobin Dissociation Revisited

Megan K. M. Young, Theodore J. Gries

• The interaction between oxygen and hemoglobin is a classic example of a cooperative ligand-binding process. This gaseous-ligand binding process may be studied in the undergraduate laboratory with a variable-pressure cuvette apparatus. An updated method is described to assemble a variable-pressure cuvette apparatus composed of inexpensive supplies and equipment commonly found in the chemistry laboratory.