A Size Exclusion Chromatography Laboratory with Unknowns for Introductory Students

• Edward J. McIntee ^[1] ; Kate J. Graham ^[1] ; Edward C. Colosky ^[1] ; Henry V. Jakubowski ^[1]
  1. [1] College of Saint Benedict and Saint John's University

     College of Saint Benedict and Saint John's University

     Township of Saint Wendel, Estados Unidos

     
• Localización: Journal of chemical education, ISSN 0021-9584, Vol. 92, Nº 12, 2015, págs. 2126-2129
• Idioma: inglés
• Texto completo no disponible (Saber más ...)
• Resumen

  • Size exclusion chromatography is an important technique in the separation of biological and polymeric samples by molecular weight. While a number of laboratory experiments have been published that use this technique for the purification of large molecules, this is the first report of an experiment that focuses on purifying an unknown small molecule from a protein. Students must then determine the structure of the unknown using spectral determination.