Resumen de Using Ion Exchange Chromatography To Separate and Quantify Complex Ions
Ion exchange chromatography is an important technique in the separation of charged species, particularly in biological, inorganic, and environmental samples. In this experiment, students are supplied with a mixture of two substitution-inert complex ions. They separate the complexes by ion exchange chromatography using a �flash� technique. After taking a visible spectrum of the eluted complexes, the unknowns are identified by comparison with a table of supplied ?max values and quantified using Beer�s law. This experiment can be completed in under 4 h and is suitable for introductory and intermediate chemistry students.
