Prevention of Neutrophil Protein Oxidation With Vitamins C and E Diet Supplementation Without Affecting the Adaptive Response to Exercise

• Autores: Antoni Sureda Gomila, Miguel David Ferrer Reynes, Antonia Mestre, Josep Antoni Tur Marí, Antoni Pons Biescas
• Localización: International journal of sport nutrition and exercise metabolism, ISSN 1526-484X, ISSN-e 1543-2742, Vol. 23, Nº. 1, 2013, págs. 31-39
• Idioma: inglés
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• Resumen

  • The authors studied the effects of antioxidant diet supplementation with an almond-based beverage on neutrophil antioxidants, nitrite, and protein oxidative alterations after exercise. Fourteen trained male amateur runners were randomly assigned in a double-blind fashion to receive antioxidant supplementation (152 mg/d vitamin C and 50 mg/d vitamin E) or placebo using an almond-based beverage for 1 mo and participated in a half-marathon race. Blood samples were taken before and after the half-marathon and after 3 hr recovery. Supplementation significantly increased basal neutrophil vitamin C compared with placebo (p < .05). Exercise increased neutrophil vitamin E levels in the supplemented group and decreased vitamin C in both groups after recovery (p < .05). Neutrophil catalase and glutathione peroxidase gene expression and nitrite levels were significantly increased as result of exercise (p < .05). Nitrotyrosine and protein carbonyl derivates increased only in the placebo group after exercise (p < .05), and these values remained high at recovery. No significant differences were evidenced in caspase-3 activity and DNA damage. Antioxidant supplementation with vitamins C and E reduced the exercise-induced oxidation of proteins in neutrophils, without altering the antioxidant adaptive response, as evidenced by the increased catalase and glutathione peroxidase gene expression.