Chemical Composition of Three Polysaccharides From Gynostemma pentaphyllum and Comparison of Their Antioxidant Activity in Skeletal Muscle of Exercised Mice

• Autores: Aiping Chi, Liang Tang, Jing Zhang, Kunru Zhang
• Localización: International journal of sport nutrition and exercise metabolism, ISSN 1526-484X, ISSN-e 1543-2742, Vol. 22, Nº. 6, 2012, págs. 479-485
• Idioma: inglés
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• Resumen

  • Purpose: To investigate the effects of polysaccharide from Gynostemma pentaphyllum on antioxidant activity in skeletal muscle of mice exercised to exhaustion. Methods: Three polysaccharide fractions were obtained from G. pentaphyllum polysaccharide (GPP) and termed GPP1-a, GPP2-b, and GPP3-a. Gas chromatography (GC) and infrared spectrum of the polysaccharides were determined. The fractions were orally administrated to mice once daily for 1 wk. The exercise time to exhaustion was assessed using a forced swim test of mice after a week. The glucose, creatine phosphokinase, and lactic dehydrogenase in serum; the activity of superoxide dismutase and glutathione peroxidase; and the levels of malondialdehyde (MDA) and glycogen in muscle were determined. Results: The results of GC demonstrated that GPP1-a, GPP2-b, and GPP3-a were composed of different monosaccharides with distinct molar ratios. Infrared spectrum showed that the main typicals of GPP1-a and GPP2-b were ß-configuration and the main typical of GPP3-a was a-configuration. Among the 3 fractions of GPP, GPP1-a administration significantly prolonged exercise time to exhaustion of mice, increased glycogen level and some of antioxidant enzyme activities, and decreased MDA level in muscle. Conclusions: The mechanism by which GPP1-a prolonged exercise time to exhaustion in mice may be associated with scavenging reactive oxygen species excessively produced and further increasing glycogen levels in skeletal muscle.