Argentina
Se caracterizaron cultivares de Avena sativa (avena blanca) y Avena byzantina (avena amarilla) mediante la respuesta de los antecios a la luz ultravioleta (UV) y se determinaron los perfiles proteicos de aveninas para las fracciones de antecios fluorescentes y no fluorescentes. Se utilizó la técnica de fluorescencia y de electroforesis en gel de poliacrilamida para aveninas. Se calcularon los intervalos de confianza para el porcentaje de antecios fluorescentes y no fluorescentes. En el análisis de electroforesis se observó la presencia/ausencia de los componentes proteínicos y se determinó el perfil proteico característico en cada cultivar. Al ser expuestos a la luz UV, cada cultivar presentó en su muestra de semillas proporciones de antecios fluorescentes o no fluorescentes, manifestando tales proporciones similares electroforegramas de aveninas. El uso de la luz UV resultó limitado para indicar la especie a la cual pertenecen los cultivares de avena blanca y de avena amarilla.
The objective of this paper was to characterize cultivars of Avena sativa (white oat) and Avena byzantina (yellow oat) by testing the response of florets to ultraviolet light (UV) and by determining avenine proteinic profiles in fractions of fluorescent and non-fluorescent florets. Fluorescence and electrophoretic techniques in polyacrylamide gel were used to evaluate avenines. Confidence intervals were calculated for the percentages of fluorescent and non-fluorescent florets. Presence/absence of proteinic components was observed in electrophoretic analysis, and the characteristic proteinic profile of each cultivar was determined. Each cultivar, when exposed to UV light, presented proportions of fluorescent and non-fluorescent florets with similar avenine electrophoretic patterns. Although PAGE avenine analysis permitted the differentiation of the analyzed cultivars, it did not group/categorize them according to the species in which they are registered. UV proved to have limited importance in differentiating white and yellow oat at the species level.
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