Structural study of CUG-repeating small RNAs complexed with silencing suppressor P19

• Autores: Elizaveta Katorcha ---
• Directores de la Tesis: Lioudmila Malinina (dir. tes.)
• Lectura: En la Universidad del País Vasco - Euskal Herriko Unibertsitatea ( España ) en 2012
• Idioma: inglés
• Tribunal Calificador de la Tesis: Félix María Goñi Urcelay (presid.), Marcelo Eduardo Guerin (secret.), Josefa de Lourdes Campos Lopez (voc.), Juan Antonio Subirana Torrent (voc.), Miquel Coll Capella (voc.)
• Materias:
  • Química
    • Bioquímica
    • Química macromolecular
  • Ciencias de la vida
    • Biología molecular
• Enlaces
  • Tesis en acceso abierto en: ADDI
• Resumen

  • The study is focused on structural aspects of interaction between silencing suppressor p19 and CUG-repeating small RNAs. The work involves crystal structure determination of a protein-unbound RNA form and RNA fragments of various lengths (19, 20, 21 nucleotides) complexed with p19-suppressor. Results prove the ability of silencing suppressor p19 to bind CUG-repeating small RNAs, as well as reveal features of U•U mismatches flanked by Watson-Crick C•G base pairs in p19-bound and p19-unbound states. In addition, structural data reveal a p19 specific site for anchoring extra nucleotides in small RNAs. In general, the study extends our knowledge about the mechanism of small RNA recognition by silencing suppressor p19.